About: The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis

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About: The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1371/journal.pone.0017285
Description	The mitosomes of Giardia intestinalis are thought to be mitochondria highly-reduced in response to the oxygen-poor niche. We performed a quantitative proteomic assessment of Giardia mitosomes to increase understanding of the function and evolutionary origin of these enigmatic organelles. Mitosome-enriched fractions were obtained from cell homogenate using Optiprep gradient centrifugation. To distinguish mitosomal proteins from contamination, we used a quantitative shot-gun strategy based on isobaric tagging of peptides with iTRAQ and tandem mass spectrometry. Altogether, 638 proteins were identified in mitosome-enriched fractions. Of these, 139 proteins had iTRAQ ratio similar to that of the six known mitosomal markers. Proteins were selected for expression in Giardia to verify their cellular localizations and the mitosomal localization of 20 proteins was confirmed. The mitosomes of Giardia intestinalis are thought to be mitochondria highly-reduced in response to the oxygen-poor niche. We performed a quantitative proteomic assessment of Giardia mitosomes to increase understanding of the function and evolutionary origin of these enigmatic organelles. Mitosome-enriched fractions were obtained from cell homogenate using Optiprep gradient centrifugation. To distinguish mitosomal proteins from contamination, we used a quantitative shot-gun strategy based on isobaric tagging of peptides with iTRAQ and tandem mass spectrometry. Altogether, 638 proteins were identified in mitosome-enriched fractions. Of these, 139 proteins had iTRAQ ratio similar to that of the six known mitosomal markers. Proteins were selected for expression in Giardia to verify their cellular localizations and the mitosomal localization of 20 proteins was confirmed. (en)
Title	The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis (en)
skos:prefLabel	The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis The Minimal Proteome in the Reduced Mitochondrion of the Parasitic Protist Giardia intestinalis (en)
skos:notation	RIV/00216208:11310/11:10082564!RIV12-GA0-11310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA204/06/0947), P(GD204/05/H023), P(LC07032), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Doležal, Pavel Hrdý, Ivan Campo Beltran, Neritza Šedinová, Miroslava Rada, Petr Jedelský, Petr Tachezy, Jan Šmíd, Ondřej Voleman, Luboš Marcinčiková, Michaela Pyrih, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	212601
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/11:10082564
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	iTRAQ; LOPIT; optiprep; mitosome; Giardia intestinalis (en)
http://linked.open.../riv/klicoveSlovo	iTRAQ Giardia intestinalis LOPIT mitosome optiprep
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B53E38B97AB0]
http://linked.open...i/riv/nazevZdroje	PLoS ONE
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	11 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Functional Genetics Origin and function of Giardia intestinalis mitosome Molecular biology of differentiating cell
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Campo Beltran, Neritza Doležal, Pavel Hrdý, Ivan Jedelský, Petr Perry, Andrew Rada, Petr Tachezy, Jan Šedinová, Miroslava Šmíd, Ondřej Marcinčiková, Michaela Pyrih, Jan Trevor, Lithgow Voleman, Luboš
http://linked.open...ain/vavai/riv/wos	000287761700043
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	1932-6203
number of pages	17 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0017285
http://localhost/t...ganizacniJednotka	11310

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