About: Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene. Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Transgenes can be silenced long after their integration. To study the long-term changes in transgene expression in potato, the activity of two reporter genes, encoding GFP and NPTII, was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro. Complete silencing of the reporter genes was observed in four lines (nearly 25 %), all of which successively silenced the two reporter genes, indicating an interconnection between their silencing. We suggest a hypothetical mechanism involving the successive silencing of the two reporter genes that involves the switch of GFP silencing from the post-transcriptional to transcriptional level and subsequent spreading of methylation to the NPTII gene. (en)
Title	Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation (en)
skos:prefLabel	Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation (en)
skos:notation	RIV/00216208:11310/10:10082159!RIV11-MSM-11310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06004), P(LC06034), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Nocarová, Eva Fischer, Lukáš Opatrný, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	290997
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/10:10082159
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GFP; potato (Solanum tuberosum); transgene silencing (en)
http://linked.open.../riv/klicoveSlovo	GFP potato (Solanum tuberosum) transgene silencing
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[5D513A2DA03B]
http://linked.open...i/riv/nazevZdroje	Annals of Botany
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Integration of research activities to study the plant genome Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	106
http://linked.open...iv/tvurceVysledku	Fischer, Lukáš Opatrný, Zdeněk Nocarová, Eva
http://linked.open...ain/vavai/riv/wos	000282167400006
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0305-7364
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	11310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 31 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software