About: Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this work was to develop a method for determining free estriol available to capacitating sperm. In order to determine a status of estriol during mouse sperm capacitation in vitro a high performance liquid chromatography HPLC method with UV detection was used. A free estriol, and the estriol bound to the bovine serum albumin in capacitation medium can be quantified by the proposed method. A reversed-phase separation mode using a SunFire C18 column with a simple mobile phase composed of acetonitrile and water, methanol and water at the ratio 40/60 (v/v) was applied. Our results show that the level of free estriol available for mouse spermatozoa during capacitation in vitro can be quantified by HPLC method with UV detection. Therefore, this method represents an important tool to determine the amount of environmental estrogens, such as estriol, bound to sperm cells at the specific time point of capacitation in vitro. The aim of this work was to develop a method for determining free estriol available to capacitating sperm. In order to determine a status of estriol during mouse sperm capacitation in vitro a high performance liquid chromatography HPLC method with UV detection was used. A free estriol, and the estriol bound to the bovine serum albumin in capacitation medium can be quantified by the proposed method. A reversed-phase separation mode using a SunFire C18 column with a simple mobile phase composed of acetonitrile and water, methanol and water at the ratio 40/60 (v/v) was applied. Our results show that the level of free estriol available for mouse spermatozoa during capacitation in vitro can be quantified by HPLC method with UV detection. Therefore, this method represents an important tool to determine the amount of environmental estrogens, such as estriol, bound to sperm cells at the specific time point of capacitation in vitro. (en)
Title	Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method (en)
skos:prefLabel	Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method (en)
skos:notation	RIV/00216208:11310/09:10000954!RIV10-MSM-11310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06011), Z(MSM0021620828), Z(MSM0021620857)
http://linked.open...iv/cisloPeriodika	Suppl 1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Frolíková, Michaela Bosáková, Zuzana Tesařová, Eva Dvořáková-Hortová, Kateřina Vašinová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	339124
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/09:10000954
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	endocrine disruptors; estriol; sperm; capacitation; HPLC (en)
http://linked.open.../riv/klicoveSlovo	capacitation sperm estriol HPLC endocrine disruptors
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E6F510CDBE5D]
http://linked.open...i/riv/nazevZdroje	FOLIA ZOOLOGICA
http://linked.open...in/vavai/riv/obor	EG
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Bosáková, Zuzana Frolíková, Michaela Tesařová, Eva Hortová, Kateřina Vašinová, Jitka Honetschlaegerova, Marie
http://linked.open...ain/vavai/riv/wos	000272048200008
http://linked.open...n/vavai/riv/zamer	Ekologické procesy v evoluci modelových skupin organismů Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí
issn	0139-7893
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	11310

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