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rdf:type
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Description
| - Trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for H-2 production. Pyruvate ferredoxin oxidoreductase and NADH dehydrogenase (NADH-DH) are the two enzymes catalyzing the production of reduced ferredoxin. The genes encoding the two subunits of NADH-DH were cloned and expressed in Escherichia coli. Kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. The recombinant holoenzyme contained 2.15 non-heme iron and 1.95 acid-labile sulfur atoms per heterodimer. The EPR spectrum of the dithionite-reduced protein revealed a [2Fe-2S] cluster with a rhombic symmetry of g(xyz)=1.917, 1.951, and 2.009 corresponding to cluster N1a of the respiratory complex I. Based on the Fe content, absorption spectrum, and the EPR spectrum of the purified small subunit, the [2Fe-2S] cluster was located in the small subunit of the holoenzyme. This recombinant NADH-DH oxidized NADH
- Trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for H-2 production. Pyruvate ferredoxin oxidoreductase and NADH dehydrogenase (NADH-DH) are the two enzymes catalyzing the production of reduced ferredoxin. The genes encoding the two subunits of NADH-DH were cloned and expressed in Escherichia coli. Kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. The recombinant holoenzyme contained 2.15 non-heme iron and 1.95 acid-labile sulfur atoms per heterodimer. The EPR spectrum of the dithionite-reduced protein revealed a [2Fe-2S] cluster with a rhombic symmetry of g(xyz)=1.917, 1.951, and 2.009 corresponding to cluster N1a of the respiratory complex I. Based on the Fe content, absorption spectrum, and the EPR spectrum of the purified small subunit, the [2Fe-2S] cluster was located in the small subunit of the holoenzyme. This recombinant NADH-DH oxidized NADH (en)
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Title
| - Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa
- Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa (en)
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skos:prefLabel
| - Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa
- Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa (en)
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skos:notation
| - RIV/00216208:11310/09:10000735!RIV10-GA0-11310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/06/0944), Z(MSM0021620858)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/09:10000735
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Complex-i; quinone oxidoreductase; spectrophotometric determination; paracoccus-denitrificans; trichomonas-vaginalis; h-2 metabolism; cyanobacteria; expression; energy; acid (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Applied biochemistry and biotechnology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hrdý, Ivan
- Šafaříková, Lucie
- Angerhofer, Alexander
- Do, Phi Minh
- Ingram, Lonnie O.
- Shanmugam, K. T..
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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