About: Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa

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About: Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for H-2 production. Pyruvate ferredoxin oxidoreductase and NADH dehydrogenase (NADH-DH) are the two enzymes catalyzing the production of reduced ferredoxin. The genes encoding the two subunits of NADH-DH were cloned and expressed in Escherichia coli. Kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. The recombinant holoenzyme contained 2.15 non-heme iron and 1.95 acid-labile sulfur atoms per heterodimer. The EPR spectrum of the dithionite-reduced protein revealed a [2Fe-2S] cluster with a rhombic symmetry of g(xyz)=1.917, 1.951, and 2.009 corresponding to cluster N1a of the respiratory complex I. Based on the Fe content, absorption spectrum, and the EPR spectrum of the purified small subunit, the [2Fe-2S] cluster was located in the small subunit of the holoenzyme. This recombinant NADH-DH oxidized NADH Trichomonas vaginalis generates reduced ferredoxin within a unique subcellular organelle, hydrogenosome that is used as a reductant for H-2 production. Pyruvate ferredoxin oxidoreductase and NADH dehydrogenase (NADH-DH) are the two enzymes catalyzing the production of reduced ferredoxin. The genes encoding the two subunits of NADH-DH were cloned and expressed in Escherichia coli. Kinetic properties of the recombinant heterodimer were similar to that of the native enzyme from the hydrogenosome. The recombinant holoenzyme contained 2.15 non-heme iron and 1.95 acid-labile sulfur atoms per heterodimer. The EPR spectrum of the dithionite-reduced protein revealed a [2Fe-2S] cluster with a rhombic symmetry of g(xyz)=1.917, 1.951, and 2.009 corresponding to cluster N1a of the respiratory complex I. Based on the Fe content, absorption spectrum, and the EPR spectrum of the purified small subunit, the [2Fe-2S] cluster was located in the small subunit of the holoenzyme. This recombinant NADH-DH oxidized NADH (en)
Title	Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa (en)
skos:prefLabel	Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa Engineering Escherichia coli for Fermentative Dihydrogen Production: Potential Role of NADH-F Oxidoreductase from the Hydrogenosome of Anaerobic Protozoa (en)
skos:notation	RIV/00216208:11310/09:10000735!RIV10-GA0-11310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/0944), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Šafaříková, Lucie Hrdý, Ivan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	313265
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/09:10000735
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Complex-i; quinone oxidoreductase; spectrophotometric determination; paracoccus-denitrificans; trichomonas-vaginalis; h-2 metabolism; cyanobacteria; expression; energy; acid (en)
http://linked.open.../riv/klicoveSlovo	quinone oxidoreductase acid cyanobacteria energy expression spectrophotometric determination trichomonas-vaginalis Complex-i h-2 metabolism paracoccus-denitrificans
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2F32DEE204F3]
http://linked.open...i/riv/nazevZdroje	Applied biochemistry and biotechnology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Trichomonad hydrogenosomes, electrontransporting pathways and drug resistance
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	153
http://linked.open...iv/tvurceVysledku	Hrdý, Ivan Šafaříková, Lucie Angerhofer, Alexander Do, Phi Minh Ingram, Lonnie O. Shanmugam, K. T..
http://linked.open...ain/vavai/riv/wos	000268308000005
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0273-2289
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	11310

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