About: Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates

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About: Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Prp45 is an yeast ortholog of Human transcription co-regulator SNW1/SKIP, which is implicated in the regulation of both transcription elongation and alternative splicing. We characterize a temperature sensitive allele of PRP45 using a synthetic lethality screen, TAP purification and primer extension splicing assay. We found that prp45(1?169) genetically interacts spliceosomal components SYF1, CLF1/SYF3, NTC20, and CEF1, SLU7, PRP17, PRP18, and PRP22. Spliceosomal complexes purified from prp45(1?169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. Splicing of canonical intron was unimpeded, wheras mutants showed elevated levels of lariatexon intermediate. We suggest that Prp45 contributes to splicing efficiency of substrates non-conforming to the consensus. Prp45 is an yeast ortholog of Human transcription co-regulator SNW1/SKIP, which is implicated in the regulation of both transcription elongation and alternative splicing. We characterize a temperature sensitive allele of PRP45 using a synthetic lethality screen, TAP purification and primer extension splicing assay. We found that prp45(1?169) genetically interacts spliceosomal components SYF1, CLF1/SYF3, NTC20, and CEF1, SLU7, PRP17, PRP18, and PRP22. Spliceosomal complexes purified from prp45(1?169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. Splicing of canonical intron was unimpeded, wheras mutants showed elevated levels of lariatexon intermediate. We suggest that Prp45 contributes to splicing efficiency of substrates non-conforming to the consensus. (en)
Title	Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates (en)
skos:prefLabel	Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates Prp45 Affects Prp22 Partition in Spliceosomal Complexes and Splicing Efficiency of Non-Consensus Substrates (en)
skos:notation	RIV/00216208:11310/09:00013036!RIV10-GA0-11310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA204/02/1512), P(LC07032), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Abrhámová, Kateřina Skružný, Michal Folk, Petr Půta, František Gahura, Ondřej Valentová, Anna Munzarová, Vanda
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	337431
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/09:00013036
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pre-mRNA PROCESSING; SACCHAROMYCES CEREVISIAE; Prp45; Prp22; SNW1/SKIP; pre-messenger-rna; receptor-mediated transcription; cell-cycle progression; 2nd step; interacting protein (en)
http://linked.open.../riv/klicoveSlovo	Prp22 Prp45 interacting protein 2nd step SACCHAROMYCES CEREVISIAE SNW1/SKIP cell-cycle progression pre-mRNA PROCESSING pre-messenger-rna receptor-mediated transcription
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[DF6AA72E55FB]
http://linked.open...i/riv/nazevZdroje	Journal of Cellular Biochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Functional Genetics The mechanism whereby the novel coactivator SNW regulates gene expression
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	106
http://linked.open...iv/tvurceVysledku	Folk, Petr Půta, František Gahura, Ondřej Valentová, Anna Munzarová, Vanda Abrhámová, Kateřina Skružný, Michal
http://linked.open...ain/vavai/riv/wos	000262238600016
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0730-2312
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	11310

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