About: New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972

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About: New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300553/full
Description	Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related macular degeneration. Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related macular degeneration. (en)
Title	New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 (en)
skos:prefLabel	New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 (en)
skos:notation	RIV/00216208:11160/13:10191186!RIV14-MSM-11160___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0061), P(NT14265), S
http://linked.open...iv/cisloPeriodika	23
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kasalová, Eva Solich, Petr Krčmová, Lenka Aufartová, Jana Plíšek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	91517
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/13:10191186
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Vitamin D; Standard reference material 972; Mass spectrometry; Clinical research (en)
http://linked.open.../riv/klicoveSlovo	Standard reference material 972 Mass spectrometry Clinical research Vitamin D
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[171F43F937C4]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	FB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Increasing of the R&D capacity at Charles University through new positions for graduates of doctoral studies Utilization of Ultra High Performance Liquid Chromatography with mass spectrometry detection for determination of vitamin D and it’s metabolites for clinical practice
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	36
http://linked.open...iv/tvurceVysledku	Plíšek, Jiří Sobotka, Luboš Solich, Petr Solichová, Dagmar Aufartová, Jana Esponda, Sarah M. Kasalová, Eva Kujovská Krčmová, Lenka Morales, Tanausú V. Oros, Roman Santana-Rodriguez, Jose J.
http://linked.open...ain/vavai/riv/wos	000327556300008
issn	1615-9306
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jssc.201300553
http://localhost/t...ganizacniJednotka	11160

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