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Description
| - Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related macular degeneration.
- Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related macular degeneration. (en)
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Title
| - New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972
- New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 (en)
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skos:prefLabel
| - New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972
- New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972 (en)
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skos:notation
| - RIV/00216208:11160/13:10191186!RIV14-MSM-11160___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - I, P(EE2.3.30.0061), P(NT14265), S
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11160/13:10191186
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Vitamin D; Standard reference material 972; Mass spectrometry; Clinical research (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Separation Science
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Plíšek, Jiří
- Sobotka, Luboš
- Solich, Petr
- Solichová, Dagmar
- Aufartová, Jana
- Esponda, Sarah M.
- Kasalová, Eva
- Kujovská Krčmová, Lenka
- Morales, Tanausú V.
- Oros, Roman
- Santana-Rodriguez, Jose J.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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http://localhost/t...ganizacniJednotka
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