About: Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0021967312013386
Description	The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC-MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision. The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC-MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision. (en)
Title	Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis (en)
skos:prefLabel	Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis (en)
skos:notation	RIV/00216208:11160/13:10146057!RIV14-MSM-11160___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	May
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Nováková, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	64880
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/13:10146057
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Method validation; Matrix effects; LC-MS; UHPLC; Sample preparation; Bioanalysis (en)
http://linked.open.../riv/klicoveSlovo	LC-MS UHPLC Bioanalysis Matrix effects Method validation Sample preparation
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C129E2ABD239]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	1292
http://linked.open...iv/tvurceVysledku	Nováková, Lucie
http://linked.open...ain/vavai/riv/wos	000319237100004
issn	0021-9673
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chroma.2012.08.087
http://localhost/t...ganizacniJednotka	11160

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