About: High-resolution peptide separations using nano-LC at ultra-high pressure

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About: High-resolution peptide separations using nano-LC at ultra-high pressure Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/jssc.201201087/full
Description	We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 mu m id capillary columns packed with 2, 3, and 5 mu m fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 mu m particle-packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic-performance limit, i.e. using 2-mu m packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run-to-run retention time stability (RSD values below 0.18%) was demonstrated applying ultra-high pressure conditions. We report on the optimization of nano-LC gradient separations of proteomic samples that vary in complexity. The gradient performance limits were visualized by kinetic plots depicting the gradient time needed to achieve a certain peak capacity, while using the maximum system pressure of 80 MPa. The selection of the optimal particle size/column length combination and corresponding gradient steepness was based on scouting the performance of 75 mu m id capillary columns packed with 2, 3, and 5 mu m fully porous silica C18 particles. At optimal gradient conditions, peak capacities up to 500 can be obtained within a 120 min gradient using 2 mu m particle-packed capillary columns. Separations of proteomic samples including a cytochrome c tryptic digest, a bovine serum albumin tryptic digest, a six protein mix digest, and an Escherichia coli digest are demonstrated while operating at the kinetic-performance limit, i.e. using 2-mu m packed columns, adjusting the column length and scaling the gradient steepness according to sample complexity. Finally, good run-to-run retention time stability (RSD values below 0.18%) was demonstrated applying ultra-high pressure conditions. (en)
Title	High-resolution peptide separations using nano-LC at ultra-high pressure High-resolution peptide separations using nano-LC at ultra-high pressure (en)
skos:prefLabel	High-resolution peptide separations using nano-LC at ultra-high pressure High-resolution peptide separations using nano-LC at ultra-high pressure (en)
skos:notation	RIV/00216208:11160/13:10146055!RIV14-MSM-11160___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Nováková, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	77431
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/13:10146055
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ultra-high pressures; Proteomics; Packed capillary columns; Nano flow rate; Kinetic plot; Column coupling (en)
http://linked.open.../riv/klicoveSlovo	Proteomics Column coupling Kinetic plot Nano flow rate Packed capillary columns Ultra-high pressures
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E240F0E4C8FC]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	36
http://linked.open...iv/tvurceVysledku	Nováková, Lucie Broeckhoven, Ken De Pra, Mauro Desmet, Gert Eeltink, Sebastiaan Stassen, Catherine Swart, Remco Vaast, Axel
http://linked.open...ain/vavai/riv/wos	000319865700005
issn	1615-9306
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jssc.201201087
http://localhost/t...ganizacniJednotka	11160

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