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| Description
| - A simple and automated HPLC column-switching method with rapid sample pretreatment has been developed for quantitative determination of beta-carotene in food supplements. Commercially samples of food supplements were dissolved in chloroform with help of saponification with 1 M solution of sodium hydroxide in ultrasound bath. A 20-min sample dissolution/extraction step was necessary before chromatography analysis to transfer beta-carotene from solid state of food supplements preparations (capsules, tablets) to chloroform solution. Sample volume - 3 mu L of chloroform phase was directly injected into the HPLC system. Next on-line sample clean-up was achieved on the pretreatment precolumn Chromolith Guard Cartridge RP-18e (Merck), 10 x 4.6 mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rate of 1.5 mL/min. Valve switch to analytical column was set at 2.5 min in a back-flush mode. After column switching to the analytical column Ascentis Express C-18, 30 x 4.6 mm, particle size 2.7 mu m (Sigma Aldrich), the separation and determination of beta-carotene in food supplements was performed using a mobile phase consisting of 100% methanol, column temperature at 60 degrees C and flow rate 1.5 mL/min. The detector was set at 450 nm. Under the optimum chromatographic conditions standard calibration curve was measured with good linearity - correlation coefficient for beta-carotene (r(2) = 0.999014; n = 6) between the peak areas and concentration of beta-carotene 20-200 mu g/mL. Accuracy of the method defined as a mean recovery was in the range 96.66-102.40%. The intraday method precision was satisfactory at three concentration levels 20, 125 and 200 mu g/mL and relative standard deviations were in the range 0.90-1.02%. The chromatography method has shown high sample throughput during column-switching pretreatment process and analysis in one step in short time (6 min) of the whole chromatographic analysis.
- A simple and automated HPLC column-switching method with rapid sample pretreatment has been developed for quantitative determination of beta-carotene in food supplements. Commercially samples of food supplements were dissolved in chloroform with help of saponification with 1 M solution of sodium hydroxide in ultrasound bath. A 20-min sample dissolution/extraction step was necessary before chromatography analysis to transfer beta-carotene from solid state of food supplements preparations (capsules, tablets) to chloroform solution. Sample volume - 3 mu L of chloroform phase was directly injected into the HPLC system. Next on-line sample clean-up was achieved on the pretreatment precolumn Chromolith Guard Cartridge RP-18e (Merck), 10 x 4.6 mm, with a washing mobile phase (methanol:water, 92:8, (v/v)) at a flow rate of 1.5 mL/min. Valve switch to analytical column was set at 2.5 min in a back-flush mode. After column switching to the analytical column Ascentis Express C-18, 30 x 4.6 mm, particle size 2.7 mu m (Sigma Aldrich), the separation and determination of beta-carotene in food supplements was performed using a mobile phase consisting of 100% methanol, column temperature at 60 degrees C and flow rate 1.5 mL/min. The detector was set at 450 nm. Under the optimum chromatographic conditions standard calibration curve was measured with good linearity - correlation coefficient for beta-carotene (r(2) = 0.999014; n = 6) between the peak areas and concentration of beta-carotene 20-200 mu g/mL. Accuracy of the method defined as a mean recovery was in the range 96.66-102.40%. The intraday method precision was satisfactory at three concentration levels 20, 125 and 200 mu g/mL and relative standard deviations were in the range 0.90-1.02%. The chromatography method has shown high sample throughput during column-switching pretreatment process and analysis in one step in short time (6 min) of the whole chromatographic analysis. (en)
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| Title
| - A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements
- A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements (en)
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| skos:prefLabel
| - A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements
- A rapid HPLC column switching method for sample preparation and determination of beta-carotene in food supplements (en)
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| skos:notation
| - RIV/00216208:11160/13:10146037!RIV14-MSM-11160___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11160/13:10146037
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Food supplements; HPLC; Column-switching; On-line sample preparation; beta-carotene (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Solich, Petr
- Šatínský, Dalibor
- Brabcová, Ivana
- Hlaváčková, Markéta
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1016/j.foodchem.2013.04.063
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| http://localhost/t...ganizacniJednotka
| |
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