About: HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development

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About: HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.future-science.com/doi/full/10.4155/bio.13.217
Description	Background: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC-MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far. Methodology: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC-ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach. Results: The advantages and drawbacks of the HILIC and reversed-phase chromatographic modes were compared and discussed in detail. Conclusion: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied. Background: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC-MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far. Methodology: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC-ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach. Results: The advantages and drawbacks of the HILIC and reversed-phase chromatographic modes were compared and discussed in detail. Conclusion: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied. (en)
Title	HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development (en)
skos:prefLabel	HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development HILIC UHPLC-MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development (en)
skos:notation	RIV/00216208:11160/13:10145994!RIV14-MSM-11160___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0022)
http://linked.open...iv/cisloPeriodika	19
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Solich, Petr Nováková, Lucie Vlčková, Hana Havlíková, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	77450
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/13:10145994
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	strategy; extraction; quantification; phospholipids; human plasma; protein precipitation; hydrophilic interaction chromatography; ultra high performance liquid chromatography; tandem mass spectrometry (en)
http://linked.open.../riv/klicoveSlovo	ultra high performance liquid chromatography extraction strategy tandem mass spectrometry quantification human plasma phospholipids hydrophilic interaction chromatography protein precipitation
http://linked.open...odStatuVydavatele	PT - Portugalská republika
http://linked.open...ontrolniKodProRIV	[89C4B959092F]
http://linked.open...i/riv/nazevZdroje	Bioanalysis
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Support of establishment, development, and mobility of quality research teams at the Charles University
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	5
http://linked.open...iv/tvurceVysledku	Solich, Petr Nováková, Lucie Vlčková, Hana Havlíková, Lucie
http://linked.open...ain/vavai/riv/wos	000324909800009
issn	1757-6180
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.4155/bio.13.217
http://localhost/t...ganizacniJednotka	11160

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