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Description
  • Ascorbic acid (AA) and Dehydroascorbic acid (DHA) belong to the group of small polar molecules, which are difficult to retain in conventional reversed phase (RP) chromatographic systems. A development of simple and fast analytical method for the simultaneous determination of both acids is a real analytical challenge. So far, most of the published methods include an oxidation/reduction pretreatment step (in order to obtain only one of redox forms) and subsequent two step analysis followed by subtraction. Such approach is however time-consuming and might also bring sever inaccuracies.
  • Ascorbic acid (AA) and Dehydroascorbic acid (DHA) belong to the group of small polar molecules, which are difficult to retain in conventional reversed phase (RP) chromatographic systems. A development of simple and fast analytical method for the simultaneous determination of both acids is a real analytical challenge. So far, most of the published methods include an oxidation/reduction pretreatment step (in order to obtain only one of redox forms) and subsequent two step analysis followed by subtraction. Such approach is however time-consuming and might also bring sever inaccuracies. (en)
Title
  • Practical problems of simultaneous determination of ascorbic and dehydroascorbic acids by HPLC
  • Practical problems of simultaneous determination of ascorbic and dehydroascorbic acids by HPLC (en)
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  • Practical problems of simultaneous determination of ascorbic and dehydroascorbic acids by HPLC
  • Practical problems of simultaneous determination of ascorbic and dehydroascorbic acids by HPLC (en)
skos:notation
  • RIV/00216208:11160/11:10100131!RIV12-MSM-11160___
http://linked.open...avai/riv/aktivita
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  • Z(MSM0021620822)
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  • 1
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  • 222402
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  • RIV/00216208:11160/11:10100131
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  • retention; stability; detection; HPLC; dehydroascorbic acid; ascorbic acid (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
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  • [093B6F12E882]
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  • Acta Universitatis Palackianae Olomucensis, Facultas rerum naturalium, Chemica
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  • 48S
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  • Nováková, Lucie
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  • 0232-0061
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  • 11160
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