About: Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays

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About: Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study we compared the potential of five commonly employed cytotoxicity assays (WST-1, XTT, MTT, Brilliant blue and Neutral red assay) to detect anti-proliferative effects of three selenium compounds, sodium selenite, seleno-L-methionine (SeMet) and Se-(Methyl)selenocysteine (SeMCys) on three colorectal cancer cell lines in vitro. Cells were exposed to the selected selenium compounds in the concentration range of 0-256 uM during 48 hours. WST-1 and XTT failed to detect cytotoxic effect. Conversely, the metabolic activity of selenium treated cells measured by WST-1 and XTT significantly increased in comparison to untreated controls. MTT, Neutral red and Brilliant blue assays were more sensitive and yielded mutually comparable results, with significant decrease of measured parameters in a concentration-dependent manner. To a smaller extent, the results were affected by the different chemical nature of the selenium compounds tested as well as by the biological properties of individual cell lines. In this study we compared the potential of five commonly employed cytotoxicity assays (WST-1, XTT, MTT, Brilliant blue and Neutral red assay) to detect anti-proliferative effects of three selenium compounds, sodium selenite, seleno-L-methionine (SeMet) and Se-(Methyl)selenocysteine (SeMCys) on three colorectal cancer cell lines in vitro. Cells were exposed to the selected selenium compounds in the concentration range of 0-256 uM during 48 hours. WST-1 and XTT failed to detect cytotoxic effect. Conversely, the metabolic activity of selenium treated cells measured by WST-1 and XTT significantly increased in comparison to untreated controls. MTT, Neutral red and Brilliant blue assays were more sensitive and yielded mutually comparable results, with significant decrease of measured parameters in a concentration-dependent manner. To a smaller extent, the results were affected by the different chemical nature of the selenium compounds tested as well as by the biological properties of individual cell lines. (en)
Title	Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays (en)
skos:prefLabel	Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays Antiproliferative effects of selenium compounds in colon cancer cells: Comparison of different cytotoxicity assays (en)
skos:notation	RIV/00216208:11160/09:00210107!RIV10-GA0-11160___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GP301/06/P047), S, Z(MSM0021620820)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Voráčová, Adéla Hašková, Pavlína
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	303525
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/09:00210107
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	selenium compounds; colon cancer; XTT; MTT; WST-1; neutral red; brilliant blue (en)
http://linked.open.../riv/klicoveSlovo	WST-1 XTT brilliant blue neutral red selenium compounds colon cancer MTT
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[29BD58CC4C8B]
http://linked.open...i/riv/nazevZdroje	Toxicology in Vitro
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Influence of inositol hexaphosphate, inositol and sodium selenite on proliferation and apoptosis colorectal carcinoma cells
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Hašková, Pavlína Rudolf, Emil Červinka, Miroslav Králová, Věra Schröterová, Ladislava Voráčová, Adéla
http://linked.open...ain/vavai/riv/wos	000271171500027
http://linked.open...n/vavai/riv/zamer	The use of experimental and clinical models of metabolic processes, nutrition and pharmacotherapy for the advancement of knowledge, clinical practice and quality of life improvement
issn	0887-2333
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	11160

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