| Attributes | Values |
|---|
| rdf:type
| |
| rdfs:seeAlso
| |
| Description
| - Background: Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress. Aims: In our study, we tested the effect of epigallocatechin gallate (EGCG), a green tea antioxidant, on the early phase of liver regeneration after PHx. Methods: Male Wistar rats were divided into 5 groups: I) laparotomy + water for injections intraperitoneally, II) laparotomy + EGCG 50 mg/kg body weight, III) PHx + water for injections, IV) PHx + EGCG 20 mg/kg, V) PHx + EGCG 50 mg/kg, during 3 consecutive days. The rats were sacrificed 24 hours after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxyline-eosine and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of genes Nqo-1 and HO-1 at mRNA level, expression of genes p21, p-p27 and p-p53 at the protein level. Results: We observed lower accumulation of BrdU in group V when compared to groups III and IV. The activity of caspases 3/7 and expression of the p-p53 were lower in group V than in groups III and IV. Tissue levels of IL-6 were lower in group V when compared to group III. Significant differences were not noted in other parameters. Conclusions: Administration of EGCG did not stimulate early phase of liver regeneration in rats after PHx. There was even lower DNA synthesis in group treated with high dose of EGCG.
- Background: Two-thirds partial hepatectomy (PHx) is an established model for the study of liver regeneration after resection. This process is accompanied by oxidative stress. Aims: In our study, we tested the effect of epigallocatechin gallate (EGCG), a green tea antioxidant, on the early phase of liver regeneration after PHx. Methods: Male Wistar rats were divided into 5 groups: I) laparotomy + water for injections intraperitoneally, II) laparotomy + EGCG 50 mg/kg body weight, III) PHx + water for injections, IV) PHx + EGCG 20 mg/kg, V) PHx + EGCG 50 mg/kg, during 3 consecutive days. The rats were sacrificed 24 hours after surgery. Biochemical analysis of rat sera was performed. Histological samples were stained with hematoxyline-eosine and bromodeoxyuridine (BrdU). In hepatectomized rats, we also measured plasma malondialdehyde, tissue malondialdehyde, glutathione and cytokines levels, the activity of caspases 3/7, expression of genes Nqo-1 and HO-1 at mRNA level, expression of genes p21, p-p27 and p-p53 at the protein level. Results: We observed lower accumulation of BrdU in group V when compared to groups III and IV. The activity of caspases 3/7 and expression of the p-p53 were lower in group V than in groups III and IV. Tissue levels of IL-6 were lower in group V when compared to group III. Significant differences were not noted in other parameters. Conclusions: Administration of EGCG did not stimulate early phase of liver regeneration in rats after PHx. There was even lower DNA synthesis in group treated with high dose of EGCG. (en)
|
| Title
| - Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats
- Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats (en)
|
| skos:prefLabel
| - Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats
- Epigallocatechin Gallate Does Not Accelerate the Early Phase of Liver Regeneration After Partial Hepatectomy in Rats (en)
|
| skos:notation
| - RIV/00216208:11150/14:10210604!RIV15-MSM-11150___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/14:10210604
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Oxidative stress; Liver regeneration; Hepatectomy; Epigallocatechin gallate; Antioxidants (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Digestive Diseases and Sciences
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kučera, Otto
- Červinková, Zuzana
- Moravcová, Alena
- Mezera, Vojtěch
- Peterová, Eva
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1007/s10620-013-2966-5
|
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)