About: Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil

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About: Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1002/cne.23181
Description	Although the extracellular space in the neuropil of the brain is an important channel for volume communication between cells and has other important functions, its morphology on the micron scale has not been analyzed quantitatively owing to experimental limitations. We used manual and computational techniques to reconstruct the 3D geometry of 180 mu m3 of rat CA1 hippocampal neuropil from serial electron microscopy and corrected for tissue shrinkage to reflect the in vivo state. The reconstruction revealed an interconnected network of 4080 nm diameter tunnels, formed at the junction of three or more cellular processes, spanned by sheets between pairs of cell surfaces with 1040 nm width. The tunnels tended to occur around synapses and axons, and the sheets were enriched around astrocytes. Monte Carlo simulations of diffusion within the reconstructed neuropil demonstrate that the rate of diffusion of neurotransmitter and other small molecules was slower in sheets than in tunnels. Thus, the non-uniformity found in the extracellular space may have specialized functions for signaling (sheets) and volume transmission (tunnels). Although the extracellular space in the neuropil of the brain is an important channel for volume communication between cells and has other important functions, its morphology on the micron scale has not been analyzed quantitatively owing to experimental limitations. We used manual and computational techniques to reconstruct the 3D geometry of 180 mu m3 of rat CA1 hippocampal neuropil from serial electron microscopy and corrected for tissue shrinkage to reflect the in vivo state. The reconstruction revealed an interconnected network of 4080 nm diameter tunnels, formed at the junction of three or more cellular processes, spanned by sheets between pairs of cell surfaces with 1040 nm width. The tunnels tended to occur around synapses and axons, and the sheets were enriched around astrocytes. Monte Carlo simulations of diffusion within the reconstructed neuropil demonstrate that the rate of diffusion of neurotransmitter and other small molecules was slower in sheets than in tunnels. Thus, the non-uniformity found in the extracellular space may have specialized functions for signaling (sheets) and volume transmission (tunnels). (en)
Title	Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil (en)
skos:prefLabel	Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil Extracellular sheets and tunnels modulate glutamate diffusion in hippocampal neuropil (en)
skos:notation	RIV/00216208:11150/13:10194019!RIV14-MSM-11150___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Špaček, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	74419
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/13:10194019
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MCell; electron microscopy; shrinkage; reconstruction; extracellular space (en)
http://linked.open.../riv/klicoveSlovo	extracellular space electron microscopy reconstruction shrinkage MCell
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[51A38402669E]
http://linked.open...i/riv/nazevZdroje	Journal of Comparative Neurology
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	521
http://linked.open...iv/tvurceVysledku	Špaček, Josef Bajaj, Chandrajit L. Bartol, Thomas M. Harris, Kristen M. Kinney, Justin P. Sejnowski, Terrence J.
http://linked.open...ain/vavai/riv/wos	000312136100009
issn	0021-9967
number of pages	17 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/cne.23181
http://localhost/t...ganizacniJednotka	11150

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