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Description
| - Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45)
- Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45) (en)
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Title
| - Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method
- Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method (en)
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skos:prefLabel
| - Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method
- Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method (en)
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skos:notation
| - RIV/00216208:11150/12:10121250!RIV13-MSM-11150___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/12:10121250
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Spectrofluorometrric metod; Glutathione assay; Glutathione (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Roušar, Tomáš
- Kučera, Otto
- Lotková, Halka
- Červinková, Zuzana
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
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http://localhost/t...ganizacniJednotka
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