About: Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method

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About: Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0003269712000656
Description	Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45) Glutathione in an important tripeptide involved in a variety of cellular processes. The aim of our work was to optimize a spectrofluorometric assay for reduced glutathione based on the reaction between glutathione and o-phthalaldehyde. We found these parameters of the method: CV <3%, interasaay CV = 5.1%, recovery = 98-102%, linearity = 0-1000 uM GSH, calibration R2 = 1.00. We also aniticipated possible effect of various amino acids on the fluorescence signal, but no interference was found. We compared the optimized fluorometric method with a popular enzymatic recycling glutathione assay and found very strong correlation of results (r = 0.99, n = 45) (en)
Title	Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method (en)
skos:prefLabel	Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method Assessment of reduced glutathione: Comparison of an optimized fluorometric assay with enzymatic recycling method (en)
skos:notation	RIV/00216208:11150/12:10121250!RIV13-MSM-11150___
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Roušar, Tomáš Kremláček, Jan Červinková, Zuzana Kučera, Otto
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	123852
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/12:10121250
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Spectrofluorometrric metod; Glutathione assay; Glutathione (en)
http://linked.open.../riv/klicoveSlovo	Glutathione assay Spectrofluorometrric metod Glutathione
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A518B8076DD6]
http://linked.open...i/riv/nazevZdroje	Analytical Biochemistry
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	423
http://linked.open...iv/tvurceVysledku	Roušar, Tomáš Kučera, Otto Lotková, Halka Červinková, Zuzana
http://linked.open...ain/vavai/riv/wos	000302336100008
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	0003-2697
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ab.2012.01.030
http://localhost/t...ganizacniJednotka	11150

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