About: Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection

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About: Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://cccc.uochb.cas.cz/76/4/0277/
Description	We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the sμtaneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 x 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0 mmol/l hydrochloric acid was stable at 4 oC for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6-97.6% for GSH and 85.0-104.4% for GSSG. The linear range is 5.0-2000.0 μmol/l, with a detection limit of 2.1 μmol/l (signal-to-noise ratio = 3) for GSH and 2.0-250.0 μmol/l, with a detection limit of 0.9 μmol/l for GSSG. We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the sμtaneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 x 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0 mmol/l hydrochloric acid was stable at 4 oC for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6-97.6% for GSH and 85.0-104.4% for GSSG. The linear range is 5.0-2000.0 μmol/l, with a detection limit of 2.1 μmol/l (signal-to-noise ratio = 3) for GSH and 2.0-250.0 μmol/l, with a detection limit of 0.9 μmol/l for GSSG. (en)
Title	Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection (en)
skos:prefLabel	Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection (en)
skos:notation	RIV/00216208:11150/11:10105719!RIV12-MSM-11150___
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MSM0021627502)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kremláček, Jan Červinková, Zuzana Kučera, Otto
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	193832
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/11:10105719
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	coulometric electrochemical and fluorescence detection; liquid chromatography; glutathione stability; glutathione disulfide; Glutathione (en)
http://linked.open.../riv/klicoveSlovo	liquid chromatography coulometric electrochemical and fluorescence detection glutathione disulfide glutathione stability Glutathione
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[9707751569B5]
http://linked.open...i/riv/nazevZdroje	Collection of Czechoslovak Chemical Communications
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	76
http://linked.open...iv/tvurceVysledku	Kanďár, Roman Kučera, Otto Lotková, Halka Marková, Miroslava Červinková, Zuzana Žáková, Pavla
http://linked.open...ain/vavai/riv/wos	000289355200005
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	0010-0765
number of pages	18 (xsd:int)
http://bibframe.org/vocab/doi	10.1135/cccc2010038
http://localhost/t...ganizacniJednotka	11150

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