About: The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://fb.cuni.cz/file/5576/FB2011A0012.pdf
Description	The aim of our research was to study the effect of mitoxantrone and two protein kinase inhibitors - caffeine (inhibitor of ATM kinase) and U0126 (inhibitor of MEK1/2 kinase) on MOLT-4 and Jurkat leukaemic cell lines. In this work we show that the inhibition of MEK1/2 is associated with an increased mortality of cells after mitoxantrone (MTX) treatment. Inhibition of ATM by caffeine postponed MTX-induced cell death in MOLT-4 cells. MTX itself induced cell cycle arrest and accumulation of the cells in late S and G2/M phase. Inhibition of ATM, but not of MEK1/2, abrogated MTX-induced cell cycle arrest. Inhibition of MEK1/2 did not change MTX-induced upregulation of p53 and p21, but inhibition of ATM decreased markedly upregulation of p53 and p21 and p53 phosphorylations on serine 15 and serine 392. It can be concluded that: 1) MTX-induced phosphorylation of p53 on serine 15 and serine 392 is ATM-dependent and MEK1/2-ERK1/2 independent. 2) ATM inhibition by caffeine prevents G2 cell arrest and in p53 positive cells MOLT-4 delays the onset of MTX-induced cell death. 3) Inhibition of MEK1/2-ERK1/2 cascade potentiates cytostatic effect of MTX regardless of p53 status. The aim of our research was to study the effect of mitoxantrone and two protein kinase inhibitors - caffeine (inhibitor of ATM kinase) and U0126 (inhibitor of MEK1/2 kinase) on MOLT-4 and Jurkat leukaemic cell lines. In this work we show that the inhibition of MEK1/2 is associated with an increased mortality of cells after mitoxantrone (MTX) treatment. Inhibition of ATM by caffeine postponed MTX-induced cell death in MOLT-4 cells. MTX itself induced cell cycle arrest and accumulation of the cells in late S and G2/M phase. Inhibition of ATM, but not of MEK1/2, abrogated MTX-induced cell cycle arrest. Inhibition of MEK1/2 did not change MTX-induced upregulation of p53 and p21, but inhibition of ATM decreased markedly upregulation of p53 and p21 and p53 phosphorylations on serine 15 and serine 392. It can be concluded that: 1) MTX-induced phosphorylation of p53 on serine 15 and serine 392 is ATM-dependent and MEK1/2-ERK1/2 independent. 2) ATM inhibition by caffeine prevents G2 cell arrest and in p53 positive cells MOLT-4 delays the onset of MTX-induced cell death. 3) Inhibition of MEK1/2-ERK1/2 cascade potentiates cytostatic effect of MTX regardless of p53 status. (en)
Title	The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells (en)
skos:prefLabel	The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells The effect of ATM and ERK1/2 inhibition on mitoxantrone-induced cell death of leukaemic cells (en)
skos:notation	RIV/00216208:11150/11:10104937!RIV12-MSM-11150___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620820)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Chmelařová, Marcela Stoklasová, Alena Ćmielová, Jana Muthná, Darina Seifrtová, Martina Řezáčová, Martina Havelek, Radim
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	196180
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/11:10104937
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	leukaemia; p53; ERK1/2; ATM; Mitoxantrone (en)
http://linked.open.../riv/klicoveSlovo	p53 ATM ERK1/2 leukaemia Mitoxantrone
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[90576FF9D8AC]
http://linked.open...i/riv/nazevZdroje	Folia Biologica
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Chmelařová, Marcela Havelek, Radim Muthná, Darina Stoklasová, Alena Řezáčová, Martina Seifrtová, Martina Zemánková, Soňa Ćmielová, Jana
http://linked.open...ain/vavai/riv/wos	000290506000005
http://linked.open...n/vavai/riv/zamer	The use of experimental and clinical models of metabolic processes, nutrition and pharmacotherapy for the advancement of knowledge, clinical practice and quality of life improvement
issn	0015-5500
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	11150

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software