About: Dental pulp stem cells and their characterization

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation. Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media). Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media. We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation. Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media). Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media. (en)
Title	Dental pulp stem cells and their characterization Dental pulp stem cells and their characterization (en)
skos:prefLabel	Dental pulp stem cells and their characterization Dental pulp stem cells and their characterization (en)
skos:notation	RIV/00216208:11150/09:00208772!RIV10-MZ0-11150___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(NR9182), S, Z(MSM0021620820)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Víšek, Benjamín Ivančaková, Romana Soukup, Tomáš Mokrý, Jaroslav Kučerová, Lenka Suchánek, Jakub
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	309462
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/09:00208772
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Dental pulp; Mesenchymal stem cells; Phenotype; Proliferation activity; Viability (en)
http://linked.open.../riv/klicoveSlovo	Dental pulp Mesenchymal stem cells Phenotype Proliferation activity Viability
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[AF339A081260]
http://linked.open...i/riv/nazevZdroje	Biomedical Papers of the Faculty of Medicine of Palacky University
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Isolation, cultivation, differentiation and phenotypic characterization of dental pulp stem cells
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	153
http://linked.open...iv/tvurceVysledku	Ivančaková, Romana Mokrý, Jaroslav Soukup, Tomáš Suchánek, Jakub Kučerová, Lenka Víšek, Benjamín
http://linked.open...ain/vavai/riv/wos	000265639300006
http://linked.open...n/vavai/riv/zamer	The use of experimental and clinical models of metabolic processes, nutrition and pharmacotherapy for the advancement of knowledge, clinical practice and quality of life improvement
issn	1213-8118
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	11150

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