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| - Quantitative gene expression analysis by real-time PCR is important in several diagnostic areas, such as the detection of minimum residual disease in leukemia and the prognostic assessment of cancer patients. To address quality assurance in this technically challenging area, the European Union (EU) has funded the EQUAL project to develop methodologic external quality assessment (EQA) relevant to diagnostic and research laboratories among the EU member states. We report here the results of the EQUAL-quant program, which assesses standards in the use of TaqMan probes, one of the most widely used assays in the implementation of real-time PCR. Methods: The EQUAL-quant reagent set was developed to assess the technical execution of a standard TaqMan assay, including RNA extraction, reverse transcription, and real-time PCR quantification of target DNA copy number.
- Quantitative gene expression analysis by real-time PCR is important in several diagnostic areas, such as the detection of minimum residual disease in leukemia and the prognostic assessment of cancer patients. To address quality assurance in this technically challenging area, the European Union (EU) has funded the EQUAL project to develop methodologic external quality assessment (EQA) relevant to diagnostic and research laboratories among the EU member states. We report here the results of the EQUAL-quant program, which assesses standards in the use of TaqMan probes, one of the most widely used assays in the implementation of real-time PCR. Methods: The EQUAL-quant reagent set was developed to assess the technical execution of a standard TaqMan assay, including RNA extraction, reverse transcription, and real-time PCR quantification of target DNA copy number. (en)
- Kvantitativní stanovení genové exprese pomocí real-time PCR je důležité v mnoha diagnostických oblastech jako je např. stanovení minimální reziduální nemoci u leukémie či pro prognózu u pacientů s nádory. Evropská unie (EU) financovala program EQUAL, který měl vyvinout systém externího posouzení kvality (EQA) pro diagnostické a výzkumné laboratoře v členských zemích EU. Zde popisujeme výsledky programu EQUAL-quant, který porovnával standardy při používání TaqMan prob. Metody: vyvinutý soubor reagencií EQUAL-quant zahrnující extrakci RNA, reverzní transkripci a real-time PCR amplifikaci byl použit v 137 laboratořích ve 29 zemích. (cs)
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| Title
| - EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR
- EQUAL-quant: Mezinárodní způsob externího posuzování kvality metody real-time PCR (cs)
- EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR (en)
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| skos:prefLabel
| - EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR
- EQUAL-quant: Mezinárodní způsob externího posuzování kvality metody real-time PCR (cs)
- EQUAL-quant: An International External Quality Assessment Scheme for Real-Time PCR (en)
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| skos:notation
| - RIV/00216208:11150/06:00005069!RIV08-MSM-11150___
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| - RIV/00216208:11150/06:00005069
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| http://linked.open.../riv/klicovaSlova
| - EQUAL-quant; International; External; Quality; Assessment; Scheme; Real-Time; PCR (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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