| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways.
- Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways. (en)
- Apoptóza indukovaná síranem zinečnatým na buněčné linii HEP-2 byla sledována během 96hodinové expozice. Během prvních 48 hodin zinek vstupoval do mitochondrií a vyvolával tvorbu kyslíkových radikálů. U exponovaných buněk docházelo k mírné aktivaci p53, snížení GSH a poškození mitochondrálních funkcí. Aktivace apoptózy byla charakterizována uvolněním cytochromu C a AIF do cytoplazmy a jádra. Použití různých farmakologických inhibitorů apoptózy (antioxidanty NAC a koenzym Q, kaspázové inhibitory z-DEVD-fmk a z-VAD-fmk, cyklosporin A a bonkgreková kyselina) prokázalo, že zinek účinkuje přímo i nepřímo na mitochondrie a výsledná apoptóza je na kaspázách závislá i nezávislá. (cs)
|
| Title
| - Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria
- Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria (en)
- Zinkem indukovaná apoptóza u HEP-2 nádorových buněk: úloha oxidačního stresu a mitochondrií (cs)
|
| skos:prefLabel
| - Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria
- Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria (en)
- Zinkem indukovaná apoptóza u HEP-2 nádorových buněk: úloha oxidačního stresu a mitochondrií (cs)
|
| skos:notation
| - RIV/00216208:11150/05:00003605!RIV06-MSM-11150___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/05:00003605
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Zinc; oxidative stress; energy metabolism; mitochondria; apoptosis (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Rudolf, Emil
- Červinka, Miroslav
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)