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| Description
| - Antisense and antigene oligonucleotides (ONs) are attractive drugs for gene therapy, but major limiting factors for their routine use are inefficient cellular uptake and low accessibility to the target sites. Adding various lipophilic conjugates to the ON improves intracellular delivery as has been previously reported. We studied the cellular delivery of various ON modifications, as well as their cytosolic and nuclear distribution in mammalian Hep2-EGFP-NLS cell line. We compared uptake efficacy of ON and LNA, both conjugated with cholesterol at the 5´ end. All ONs were 3´ labeled with fluorescent Cy5 dye. We made a comparison of the ONs uptake efficacy and the kinetics, both adding ONs to the culture medium, and using streptolysin-O (SL-O) permeabilization.
- Antisense and antigene oligonucleotides (ONs) are attractive drugs for gene therapy, but major limiting factors for their routine use are inefficient cellular uptake and low accessibility to the target sites. Adding various lipophilic conjugates to the ON improves intracellular delivery as has been previously reported. We studied the cellular delivery of various ON modifications, as well as their cytosolic and nuclear distribution in mammalian Hep2-EGFP-NLS cell line. We compared uptake efficacy of ON and LNA, both conjugated with cholesterol at the 5´ end. All ONs were 3´ labeled with fluorescent Cy5 dye. We made a comparison of the ONs uptake efficacy and the kinetics, both adding ONs to the culture medium, and using streptolysin-O (SL-O) permeabilization. (en)
- Antisens a antigene oligonukleotidy (ON) jsou atraktivní látky pro genovou terapii, jejich hlavním nedostatkem z pohledu rutinního používání je neefektivní buněčný uptake a nízká dostupnost v místě působení. Přidáním různých lipofilních konjugátů k ON zlepšuje jejich buněčnou dostupnost jak bylo již dříve prokázáno. My jsme studovali buněčné vychytávání různých modiffikací ON, jejich cytosolickou a jadernou distribuci u savčí buněčné linie Hep2-EGFP-NLS. Srovnávali jsme účinnost vychytávání ON a LNA, obojí konjugované s cholesterolem na 5´ konci. Všechny ON byly na 3´konci značeny fluorescenční Cy5 barvou. Provedli jsme srovnání vychytávání a kinetiky ON jak při přidání ON do media, tak po použití permeabilizace streptolysinem-O. (cs)
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| Title
| - Cholesterol conjugated oligonucleotide and LNA: A comparison of cellular and nuclear uptake by Hep2 cells enhanced by Streptolysin-O
- Oligonukleotidy a LNA konjugované s cholesterolem: Porovnání buněčného a jaderného vychytávání u Hep-2 buněk po stimulaci Streptolysinem-O (cs)
- Cholesterol conjugated oligonucleotide and LNA: A comparison of cellular and nuclear uptake by Hep2 cells enhanced by Streptolysin-O (en)
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| skos:prefLabel
| - Cholesterol conjugated oligonucleotide and LNA: A comparison of cellular and nuclear uptake by Hep2 cells enhanced by Streptolysin-O
- Oligonukleotidy a LNA konjugované s cholesterolem: Porovnání buněčného a jaderného vychytávání u Hep-2 buněk po stimulaci Streptolysinem-O (cs)
- Cholesterol conjugated oligonucleotide and LNA: A comparison of cellular and nuclear uptake by Hep2 cells enhanced by Streptolysin-O (en)
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| skos:notation
| - RIV/00216208:11150/05:00003583!RIV06-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/05:00003583
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - gene therapy; LNA; oligonucleotide; uptake; Hep2; streptolysin-O (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Molecular and Cellular Biochemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Vokurková, Doris
- Červinka, Miroslav
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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