About: Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples     Goto   Sponge   NotDistinct   Permalink

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  • We present the results of analytical verification and our experience with clinical use of real-timePCR for relative quantification of BCR-ABL RNA transcripts. A total of 90 peripheral blood and bone marrow specimens were analysed. For real-time PCR the LightCycler t(9; 22) Quantification Kit, based on hybridization probe chemistry, was used. The number of BCR-ABL transcripts in clinical samples was normalized in ratio to the amount of G6PDH transcripts and the results were expressed in percentages. Conclusions: Despite single-step amplification, real-time PCR is a sensitive laboratory method that, compared to SQ-PCR, has provided concordant and reproducible results in clinical samples where BCR-ABL levels were higher than 0.001%.
  • We present the results of analytical verification and our experience with clinical use of real-timePCR for relative quantification of BCR-ABL RNA transcripts. A total of 90 peripheral blood and bone marrow specimens were analysed. For real-time PCR the LightCycler t(9; 22) Quantification Kit, based on hybridization probe chemistry, was used. The number of BCR-ABL transcripts in clinical samples was normalized in ratio to the amount of G6PDH transcripts and the results were expressed in percentages. Conclusions: Despite single-step amplification, real-time PCR is a sensitive laboratory method that, compared to SQ-PCR, has provided concordant and reproducible results in clinical samples where BCR-ABL levels were higher than 0.001%. (en)
  • Uvádíme výsledky analytické verifikace a naší zkušenosti s klinickým užitím 'real-time' PCR pro relativní kvantifikaci BCR-ABL RNA transkripce. Bylo analyzováno celkem 90 vzorků krve a kostní dřeně. Pro 'real-time' PCR byl použit kvantifikační set LightCycler t(9; 22), založený na chemickém způsobu studia hybridizace. Počet BCR-ABL transkripcí byl v klinických vzorcích normalizován v poměru k hodnotě G6PGH přepisů a výsledky byly vyjádřeny v procentech. Závěr: Navzdory jednokrokové amplifikaci, 'real-time' PCR je citlivá laboratorní metoda, která ve srovnání s SQ-PCR poskytuje shodné a reprodukovatelné výsledky u klinických vzorků, kde hodnoty BCR-ABL byly vyšší než 0.001%. (cs)
Title
  • Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples
  • Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples (en)
  • Kvantifikace transkriptů BCR-ABL pomocí RT-PCR u vzorků leukémie (cs)
skos:prefLabel
  • Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples
  • Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples (en)
  • Kvantifikace transkriptů BCR-ABL pomocí RT-PCR u vzorků leukémie (cs)
skos:notation
  • RIV/00216208:11150/04:00004630!RIV08-MSM-11150___
http://linked.open.../vavai/riv/strany
  • 147;151
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 583330
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11150/04:00004630
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Quantification; BCR-ABL; Transcripts; Real-Time; Leukemia; Samples (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [E39671D1D29B]
http://linked.open...i/riv/nazevZdroje
  • Klinická biochemie a metabolismus
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 12
http://linked.open...iv/tvurceVysledku
  • Beránek, Martin
  • Palička, Vladimír
  • Žák, Pavel
issn
  • 1210-7921
number of pages
http://localhost/t...ganizacniJednotka
  • 11150
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