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Description
| - We present the results of analytical verification and our experience with clinical use of real-timePCR for relative quantification of BCR-ABL RNA transcripts. A total of 90 peripheral blood and bone marrow specimens were analysed. For real-time PCR the LightCycler t(9; 22) Quantification Kit, based on hybridization probe chemistry, was used. The number of BCR-ABL transcripts in clinical samples was normalized in ratio to the amount of G6PDH transcripts and the results were expressed in percentages. Conclusions: Despite single-step amplification, real-time PCR is a sensitive laboratory method that, compared to SQ-PCR, has provided concordant and reproducible results in clinical samples where BCR-ABL levels were higher than 0.001%.
- We present the results of analytical verification and our experience with clinical use of real-timePCR for relative quantification of BCR-ABL RNA transcripts. A total of 90 peripheral blood and bone marrow specimens were analysed. For real-time PCR the LightCycler t(9; 22) Quantification Kit, based on hybridization probe chemistry, was used. The number of BCR-ABL transcripts in clinical samples was normalized in ratio to the amount of G6PDH transcripts and the results were expressed in percentages. Conclusions: Despite single-step amplification, real-time PCR is a sensitive laboratory method that, compared to SQ-PCR, has provided concordant and reproducible results in clinical samples where BCR-ABL levels were higher than 0.001%. (en)
- Uvádíme výsledky analytické verifikace a naší zkušenosti s klinickým užitím 'real-time' PCR pro relativní kvantifikaci BCR-ABL RNA transkripce. Bylo analyzováno celkem 90 vzorků krve a kostní dřeně. Pro 'real-time' PCR byl použit kvantifikační set LightCycler t(9; 22), založený na chemickém způsobu studia hybridizace. Počet BCR-ABL transkripcí byl v klinických vzorcích normalizován v poměru k hodnotě G6PGH přepisů a výsledky byly vyjádřeny v procentech. Závěr: Navzdory jednokrokové amplifikaci, 'real-time' PCR je citlivá laboratorní metoda, která ve srovnání s SQ-PCR poskytuje shodné a reprodukovatelné výsledky u klinických vzorků, kde hodnoty BCR-ABL byly vyšší než 0.001%. (cs)
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Title
| - Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples
- Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples (en)
- Kvantifikace transkriptů BCR-ABL pomocí RT-PCR u vzorků leukémie (cs)
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skos:prefLabel
| - Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples
- Quantification of BCR-ABL Transcripts by Real-Time PCR in Leukemia Samples (en)
- Kvantifikace transkriptů BCR-ABL pomocí RT-PCR u vzorků leukémie (cs)
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skos:notation
| - RIV/00216208:11150/04:00004630!RIV08-MSM-11150___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/04:00004630
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Quantification; BCR-ABL; Transcripts; Real-Time; Leukemia; Samples (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Klinická biochemie a metabolismus
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Beránek, Martin
- Palička, Vladimír
- Žák, Pavel
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number of pages
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http://localhost/t...ganizacniJednotka
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