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| rdf:type
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| Description
| - Methods of processing and cryopreservation are believed to be the most important factors of long term clinical performance of biological heart valve prostheses. That is why we decided to cooperate in evaluating the impact of current AHV (allograft heartvalve) bank protocol on valve tissue morphology. AHV harvested from 'heart-beating' cadaveric donors, considered as a fresh tissue, were compared with valve samples from non-heart beating donors, samples stored in saline, samples treated with antibiotic solution, and finally with cryopreserved valves, stored in liquid nitrogen for months. All samples were dissected, dried with hexamethyldisilazane (HMDS) method, gold-coated, studied and photographed in scanning electron microscope Tesla BS 301. Different superficial patterns were found on ventricular and vascular surfaces of „fresh“ semilunar valves. We were able to detect early changes of endothelium after harvesting, denudation of endothelial covering during preservation with and without freezing.
- Methods of processing and cryopreservation are believed to be the most important factors of long term clinical performance of biological heart valve prostheses. That is why we decided to cooperate in evaluating the impact of current AHV (allograft heartvalve) bank protocol on valve tissue morphology. AHV harvested from 'heart-beating' cadaveric donors, considered as a fresh tissue, were compared with valve samples from non-heart beating donors, samples stored in saline, samples treated with antibiotic solution, and finally with cryopreserved valves, stored in liquid nitrogen for months. All samples were dissected, dried with hexamethyldisilazane (HMDS) method, gold-coated, studied and photographed in scanning electron microscope Tesla BS 301. Different superficial patterns were found on ventricular and vascular surfaces of „fresh“ semilunar valves. We were able to detect early changes of endothelium after harvesting, denudation of endothelial covering during preservation with and without freezing. (en)
- Metody zpracování a kryoprezervace se považují za důležité faktory pro dlouhodobý klinický výkon biologické náhrady srdeční chlopně. Proto jsme se zjištovali podíl protokolu tkáňové banky alograftu srdeční chlopně (AHV) na morfologii tkáně chlopně. AHV získané z bijícího srdce kadaverózních dárců byly porovnávány s vzorky z nebijících srdcí, vzorky skladovanými ve fyziologickém roztoku, vzorky ošetřenými antibiotickým roztokem a kryoprezerovanými vzorky. Vzorky byly rozřezány, vysušeny hexamethyldisalazinem, potaženy zlatem a studovány pomocí skenovacího elektronového mikroskopu Tesla BS 301. Rozdílné povrchové vzory byly nalezeny na ventrikulárním a vaskulárním povrchu čerstvých poloměsíčitých chlopní. Detekovali jsme časné změny endotelu během uchovávání pomocí nebo bez pomoci zmražení. Věříme, že další morfologický výzkum pomůže vyvinout vhodnější protokol pro zacházení s AHV v bankách srdečních chlopní. (cs)
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| Title
| - Vliv zpracování na strukturu povrchu alogenních štěpů lidských chlopní (cs)
- Impact of processing on surface structure of human cardiac valve allografts
- Impact of processing on surface structure of human cardiac valve allografts (en)
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| skos:prefLabel
| - Vliv zpracování na strukturu povrchu alogenních štěpů lidských chlopní (cs)
- Impact of processing on surface structure of human cardiac valve allografts
- Impact of processing on surface structure of human cardiac valve allografts (en)
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| skos:notation
| - RIV/00216208:11150/04:00004608!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/04:00004608
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Impact; processing; surface; structure; human; cardiac; valve; allografts (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Acta Medica (Hradec Králové)
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Krs, Otakar
- Slížová, Dáša
- Hottmar, Petr
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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