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| Description
| - V této studii jsme hodnotili angiogenní odpovědi různých členů rodiny lidských vaskulárních endoteliálních růstových faktorů (VEGF) in vivo pomocí adenovirem zprostředkovaného přenosu. Adenoviry kódující VEGF-A, -B, -C, -D, VEGF-C N C a VEGF-D N C (N C jsou proteolyticky štěpené formy) byly přeneseny do periadventicie králičích krčních tepen límečkovou technikou. Exprese přenesených VEGF byla potvrzena imunohistochemicky a pomocí RT-PCR. 7 dní po transfekci byla pozorována maximální novotvorba cév u VEGF-A, -D a VEGF-D N C transfekovaných tepen. Okolo nových tepen byly pozorovány pericyty a intraluminální erytrocyty. Infiltrace imunitními buňkami jako odpovědi na VEGF-D a VEGF-D N C byla slabší než u jiných VEGF. V periadventicii se nevyskytla žádná tvorba lymfatických cév po transfekci všemi VEGF. (cs)
- In this study we evaluated angiogenic responses of the different members of the VEGF family in vivo using adenoviral gene transfer. Adenoviruses (1*109 plaque-forming units [pfu]) encoding for VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-CN C and VEGF-D N C (N C are proteolytically cleaved forms) were transferred locally to the periadventitial space of the rabbit carotid arteries using a collar technique that allows efficient local transfection of the periadventitial tissue. Expression of the transfected VEGFs was confirmed by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Seven days after the gene transfer maximum neovessel formation was observed in VEGF-A-, VEGF-D-, and VEGF-D N C-transfected arteries. VEGF-C N C also showed angiogenic activity whereas VEGF-B was not effective in inducing angiogenesis. Pericytes were detected around the neovessels, which also frequently showed the presence of intraluminal erythrocytes.
- In this study we evaluated angiogenic responses of the different members of the VEGF family in vivo using adenoviral gene transfer. Adenoviruses (1*109 plaque-forming units [pfu]) encoding for VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-CN C and VEGF-D N C (N C are proteolytically cleaved forms) were transferred locally to the periadventitial space of the rabbit carotid arteries using a collar technique that allows efficient local transfection of the periadventitial tissue. Expression of the transfected VEGFs was confirmed by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Seven days after the gene transfer maximum neovessel formation was observed in VEGF-A-, VEGF-D-, and VEGF-D N C-transfected arteries. VEGF-C N C also showed angiogenic activity whereas VEGF-B was not effective in inducing angiogenesis. Pericytes were detected around the neovessels, which also frequently showed the presence of intraluminal erythrocytes. (en)
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| Title
| - Angiogenní odpovědi na vaskulární endotelové růstové faktory v periadventiciální tkáni (cs)
- Angiogenic Responses of Vascular Endothelial Growth Factors in Periadventitial Tissue
- Angiogenic Responses of Vascular Endothelial Growth Factors in Periadventitial Tissue (en)
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| skos:prefLabel
| - Angiogenní odpovědi na vaskulární endotelové růstové faktory v periadventiciální tkáni (cs)
- Angiogenic Responses of Vascular Endothelial Growth Factors in Periadventitial Tissue
- Angiogenic Responses of Vascular Endothelial Growth Factors in Periadventitial Tissue (en)
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| skos:notation
| - RIV/00216208:11150/03:00004260!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/03:00004260
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Angiogenic; Responses; Vascular; Endothelial; Growth; Factors; Periadventitial; Tissue (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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