About: Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.engmech.cz/2014/im/doc/EM2014_proceedings.pdf
Description	Aims of our study were as follows: 1) to perform a 3D reconstruction of parts of the adjacent classical hepatic lobules, 2) to compare the histomorphometric techniques available for estimating the volume of the lobules using 3D reconstruction and interactive stereological techniques, 3) to assess the volume fraction of liver parenchyma, connective tissue, and blood vessels, 4) to quantify the shrinkage in tissue blocks porcine liver. The morphometric parameters were assessed using serial histological sections and stereological methods. Volume of the sampled portions of the lobules ranged between 0.282-0.498 mm3. The volume fraction of parenchyma in liver was 0.867, the volume fraction of connective tissue was 0.077and the fraction of blood vessels was 0.026. The mean volume shrinkage of porcine liver was 55.23%. This value can be used for correcting the data based on paraffin-processed histological sections. For further quantitative studies, we suggest the nucleator technique as a fast, robust and reliable method for estimating the volume of the lobules. For biomechanical modelling, the absolute volumes resulting from histological studies have to be corrected due to the tissue shrinkage affecting the paraffin-processed tissue samples. Aims of our study were as follows: 1) to perform a 3D reconstruction of parts of the adjacent classical hepatic lobules, 2) to compare the histomorphometric techniques available for estimating the volume of the lobules using 3D reconstruction and interactive stereological techniques, 3) to assess the volume fraction of liver parenchyma, connective tissue, and blood vessels, 4) to quantify the shrinkage in tissue blocks porcine liver. The morphometric parameters were assessed using serial histological sections and stereological methods. Volume of the sampled portions of the lobules ranged between 0.282-0.498 mm3. The volume fraction of parenchyma in liver was 0.867, the volume fraction of connective tissue was 0.077and the fraction of blood vessels was 0.026. The mean volume shrinkage of porcine liver was 55.23%. This value can be used for correcting the data based on paraffin-processed histological sections. For further quantitative studies, we suggest the nucleator technique as a fast, robust and reliable method for estimating the volume of the lobules. For biomechanical modelling, the absolute volumes resulting from histological studies have to be corrected due to the tissue shrinkage affecting the paraffin-processed tissue samples. (en)
Title	Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections (en)
skos:prefLabel	Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections Morphometry and reconstruction of hepatic lobules in pig based on serial histological sections (en)
skos:notation	RIV/00216208:11140/14:10284523!RIV15-MSM-11140___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0076), S
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Kubíková, Tereza Liška, Václav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Plzni
http://linked.open...dnocenehoVysledku	30571
http://linked.open...ai/riv/idVysledku	RIV/00216208:11140/14:10284523
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nucleator; point grid; volume; stereology; liver (en)
http://linked.open.../riv/klicoveSlovo	nucleator point grid volume liver stereology
http://linked.open...ontrolniKodProRIV	[E916C707E610]
http://linked.open...v/mistoKonaniAkce	Svratka, Česká republika
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Enginnering Mechanics 2014
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomedical Centre of the Faculty of Medicine in Pilsen
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Liška, Václav Tonar, Zbyněk Kubíková, Tereza Witter, Kirsti
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2014-05-12 (xsd:date)
issn	1805-8248
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	VUTB - Vysoké učení technické v Brně
https://schema.org/isbn	978-80-214-4871-1
http://localhost/t...ganizacniJednotka	11140

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