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  • Carbapenemase-producing bacteria have now spread all over the world. Infections caused by those bacteria are difficult to treat. Therefore, there is an urgent need for accurate and fast detection of carbapenemases in diagnostic laboratories. In this review, we summarize screening methods for suspected isolates, direct assays for confirmation of carbapenemase activity (e.g. the Carba NP test and matrix-assisted laser desorption ionization time-of-flight mass spectrometry carbapenem hydrolysis assay), inhibitor-based methods for carbapenemase classification, and molecular-genetic techniques for precise identification of carbapenemase genes. We also propose a workflow for carbapenemase identification in diagnostic laboratories.
  • Carbapenemase-producing bacteria have now spread all over the world. Infections caused by those bacteria are difficult to treat. Therefore, there is an urgent need for accurate and fast detection of carbapenemases in diagnostic laboratories. In this review, we summarize screening methods for suspected isolates, direct assays for confirmation of carbapenemase activity (e.g. the Carba NP test and matrix-assisted laser desorption ionization time-of-flight mass spectrometry carbapenem hydrolysis assay), inhibitor-based methods for carbapenemase classification, and molecular-genetic techniques for precise identification of carbapenemase genes. We also propose a workflow for carbapenemase identification in diagnostic laboratories. (en)
Title
  • Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories
  • Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories (en)
skos:prefLabel
  • Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories
  • Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories (en)
skos:notation
  • RIV/00216208:11140/14:10272517!RIV15-MSM-11140___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(EE2.3.30.0022)
http://linked.open...iv/cisloPeriodika
  • 9
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  • 10597
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11140/14:10272517
http://linked.open...riv/jazykVysledku
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  • MALDI-TOF; resistance; antibiotic; beta-lactamase; carbapenemase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [7956729AB57C]
http://linked.open...i/riv/nazevZdroje
  • Clinical Microbiology and Infection
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 20
http://linked.open...iv/tvurceVysledku
  • Chudáčková, Eva
  • Hrabák, Jaroslav
  • Papagiannitsis, Constantinos
http://linked.open...ain/vavai/riv/wos
  • 000343824500014
issn
  • 1198-743X
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/1469-0691.12678
http://localhost/t...ganizacniJednotka
  • 11140
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