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| rdfs:seeAlso
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| Description
| - Aquaporin-4 (AQP4) is the primary cellular water channel in the brain and is abundantly expressed by astrocytes along the blood-brain barrier and brain-cerebrospinal fluid interfaces. Water transport via AQP4 contributes to the activity-dependent volume changes of the extracellular space (ECS), which affect extracellular solute concentrations and neuronal excitability. AQP4 is anchored by alpha-syntrophin (alpha-syn), the deletion of which leads to reduced AQP4 levels in perivascular and subpial membranes. We used the real-time iontophoretic method and/or diffusion-weighted magnetic resonance imaging to clarify the impact of alpha-syn deletion on astrocyte morphology and changes in extracellular diffusion associated with cell swelling in vitro and in vivo. In mice lacking alpha-syn, we found higher resting values of the apparent diffusion coefficient of water (ADC(W)) and the extracellular volume fraction (alpha). No significant differences in tortuosity (lambda) or non-specific uptake (k'), were found between alpha-syn-negative (alpha-syn -/-) and alpha-syn-positive (alpha-syn +/+) mice. The deletion of alpha-syn resulted in a significantly smaller relative decrease in alpha observed during elevated K+ (10 mM) and severe hypotonic stress (-100 mOsmol/l), but not during mild hypotonic stress (-50 mOsmol/l). After the induction of terminal ischemia/anoxia, the final values of ADC(W) as well as of the ECS volume fraction alpha indicate milder cell swelling in alpha-syn -/- in comparison with alpha-syn +/+ mice. Shortly after terminal ischemia/anoxia induction, the onset of a steep rise in the extracellular potassium concentration and an increase in lambda was faster in alpha-syn -/- mice, but the final values did not differ between alpha-syn -/- and alpha-syn +/+ mice.
- Aquaporin-4 (AQP4) is the primary cellular water channel in the brain and is abundantly expressed by astrocytes along the blood-brain barrier and brain-cerebrospinal fluid interfaces. Water transport via AQP4 contributes to the activity-dependent volume changes of the extracellular space (ECS), which affect extracellular solute concentrations and neuronal excitability. AQP4 is anchored by alpha-syntrophin (alpha-syn), the deletion of which leads to reduced AQP4 levels in perivascular and subpial membranes. We used the real-time iontophoretic method and/or diffusion-weighted magnetic resonance imaging to clarify the impact of alpha-syn deletion on astrocyte morphology and changes in extracellular diffusion associated with cell swelling in vitro and in vivo. In mice lacking alpha-syn, we found higher resting values of the apparent diffusion coefficient of water (ADC(W)) and the extracellular volume fraction (alpha). No significant differences in tortuosity (lambda) or non-specific uptake (k'), were found between alpha-syn-negative (alpha-syn -/-) and alpha-syn-positive (alpha-syn +/+) mice. The deletion of alpha-syn resulted in a significantly smaller relative decrease in alpha observed during elevated K+ (10 mM) and severe hypotonic stress (-100 mOsmol/l), but not during mild hypotonic stress (-50 mOsmol/l). After the induction of terminal ischemia/anoxia, the final values of ADC(W) as well as of the ECS volume fraction alpha indicate milder cell swelling in alpha-syn -/- in comparison with alpha-syn +/+ mice. Shortly after terminal ischemia/anoxia induction, the onset of a steep rise in the extracellular potassium concentration and an increase in lambda was faster in alpha-syn -/- mice, but the final values did not differ between alpha-syn -/- and alpha-syn +/+ mice. (en)
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| Title
| - The Impact of Alpha-Syntrophin Deletion on the Changes in Tissue Structure and Extracellular Diffusion Associated with Cell Swelling under Physiological and Pathological Conditions
- The Impact of Alpha-Syntrophin Deletion on the Changes in Tissue Structure and Extracellular Diffusion Associated with Cell Swelling under Physiological and Pathological Conditions (en)
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| skos:prefLabel
| - The Impact of Alpha-Syntrophin Deletion on the Changes in Tissue Structure and Extracellular Diffusion Associated with Cell Swelling under Physiological and Pathological Conditions
- The Impact of Alpha-Syntrophin Deletion on the Changes in Tissue Structure and Extracellular Diffusion Associated with Cell Swelling under Physiological and Pathological Conditions (en)
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| skos:notation
| - RIV/00216208:11130/13:10209729!RIV14-GA0-11130___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(GA309/09/1597), P(GAP303/11/2378), P(GBP304/12/G069)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11130/13:10209729
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - space; k+; mice; brain; glial-cells; volume changes; potassium channel; cerebral-ischemia; rat spinal-cord; aquaporin-4 water channels (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Syková, Eva
- Vargová, Lýdia
- Voříšek, Ivan
- Cicanič, Michal
- Anděrová, Miroslava
- Dmytrenko, Lesia
- Ottersen, Ole Petter
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| http://linked.open...ain/vavai/riv/wos
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| issn
| |
| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1371/journal.pone.0068044
|
| http://localhost/t...ganizacniJednotka
| |
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