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| Description
| - The present study investigated whether oxidative stress plays a role in ischemia-reperfusion-induced changes in cardiac gene expression of Na+-K+ ATPase isoforms. The levels of mRNA for Na+-K+ ATPase isoforms were assessed in the isolated rat heart subjected to global ischemia (30 min) followed by reperfusion (60 min) in the presence or absence of superoxide dismutase (5 x 10(4) U/L) plus catalase (7.5 x 10(4) U/L), an antioxidant mixture. The levels of mRNA for the alpha(2), alpha(3), and beta(1) isoforms of Na+-K+ ATPase were significantly reduced in the ischemia-reperfusion hearts, unlike the alpha(1) isoform. Pretreatment with superoxide dismutase + catalase preserved the ischemia-reperfusion-induced changes in alpha(2), alpha(3), and beta(1) isoform mRNA levels of the Na+-K+ ATPase, whereas the alpha(1) mRNA levels were unaffected. In order to test if oxidative stress produced effects similar to those seen with ischemia-reperfusion, hearts were perfused with an oxidant, H2O2 (300 muM), or a free radical generator, xanthine (2 mM) plus xanthine oxidase (0.03 U/ml) for 20 min. Perfusion of hearts with H2O2 or xanthine/xanthine oxidase depressed the alpha(2), alpha(3), and beta(1) isoform mRNA levels of the Na+-K+ ATPase, but had lesser effects on alpha(1) mRNA levels. These results indicate that Na+-K+ ATPase isoform gene expression is altered differentially in the ischemia-reperfusion hearts and that antioxidant treatment appears to attenuate these changes. It is suggested that alterations in Na+-K+ ATPase isoform gene expression by ischemia-reperfusion may be mediated by oxidative stress
- The present study investigated whether oxidative stress plays a role in ischemia-reperfusion-induced changes in cardiac gene expression of Na+-K+ ATPase isoforms. The levels of mRNA for Na+-K+ ATPase isoforms were assessed in the isolated rat heart subjected to global ischemia (30 min) followed by reperfusion (60 min) in the presence or absence of superoxide dismutase (5 x 10(4) U/L) plus catalase (7.5 x 10(4) U/L), an antioxidant mixture. The levels of mRNA for the alpha(2), alpha(3), and beta(1) isoforms of Na+-K+ ATPase were significantly reduced in the ischemia-reperfusion hearts, unlike the alpha(1) isoform. Pretreatment with superoxide dismutase + catalase preserved the ischemia-reperfusion-induced changes in alpha(2), alpha(3), and beta(1) isoform mRNA levels of the Na+-K+ ATPase, whereas the alpha(1) mRNA levels were unaffected. In order to test if oxidative stress produced effects similar to those seen with ischemia-reperfusion, hearts were perfused with an oxidant, H2O2 (300 muM), or a free radical generator, xanthine (2 mM) plus xanthine oxidase (0.03 U/ml) for 20 min. Perfusion of hearts with H2O2 or xanthine/xanthine oxidase depressed the alpha(2), alpha(3), and beta(1) isoform mRNA levels of the Na+-K+ ATPase, but had lesser effects on alpha(1) mRNA levels. These results indicate that Na+-K+ ATPase isoform gene expression is altered differentially in the ischemia-reperfusion hearts and that antioxidant treatment appears to attenuate these changes. It is suggested that alterations in Na+-K+ ATPase isoform gene expression by ischemia-reperfusion may be mediated by oxidative stress (en)
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| Title
| - Ischemia-reperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart
- Ischemia-reperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart (en)
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| skos:prefLabel
| - Ischemia-reperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart
- Ischemia-reperfusion alters gene expression of Na+-K+ ATPase isoforms in rat heart (en)
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| skos:notation
| - RIV/00216208:11130/03:3066!RIV13-MSM-11130___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11130/03:3066
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - ischemia-reperfusion; oxidative stress; cardiac Na+-K+ ATPase isoforms; cardiac gene expression; antioxidants (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biochemical and Biophysical Research Communications
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Ošťádal, Petr
- Chapman, D.
- Dhalla, NS
- Elmoselhi, AB
- Lukas, A.
- Zdobnicka, I.
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| http://linked.open...ain/vavai/riv/wos
| |
| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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