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  • Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant.
  • Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is based on the well-established Lubbock chronic wound biofilm transferred onto an artificial agar wound bed. The biofilm formed by four bacterial species (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa) was stable for up to 48h post-transplant. The applicability of the model was evaluated by testing two common iodine wound treatments. These observations indicate that this method enables assessing the effects of treatments on established resilient wound biofilms and is clinically highly relevant. (en)
Title
  • Multispecies biofilm in an artificial wound bed: A novel model for in vitro assessment of solid antimicrobial dressings
  • Multispecies biofilm in an artificial wound bed: A novel model for in vitro assessment of solid antimicrobial dressings (en)
skos:prefLabel
  • Multispecies biofilm in an artificial wound bed: A novel model for in vitro assessment of solid antimicrobial dressings
  • Multispecies biofilm in an artificial wound bed: A novel model for in vitro assessment of solid antimicrobial dressings (en)
skos:notation
  • RIV/00216208:11120/14:43908522!RIV15-MSM-11120___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, S
http://linked.open...iv/cisloPeriodika
  • August
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 31123
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11120/14:43908522
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Multi-species wound biofilm model; In vitro testing; Artificial wound bed; Anti-biofilm substances (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [39C379DF9347]
http://linked.open...i/riv/nazevZdroje
  • Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 103
http://linked.open...iv/tvurceVysledku
  • Pavlík, Vojtěch
http://linked.open...ain/vavai/riv/wos
  • 000339150300005
issn
  • 0167-7012
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.mimet.2014.05.008
http://localhost/t...ganizacniJednotka
  • 11120
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