About: 2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

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Description	Capillary electrophoresis (CE) with contactless conductivity detection (C4D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C4D determinations of 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5 to 10 mol/L acetic acid. The time of analysis does not exceed 6 min., the limits of detection vary from 0.1 to 1.7 µmol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal and amniotic fluid samples Capillary electrophoresis (CE) with contactless conductivity detection (C4D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C4D determinations of 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5 to 10 mol/L acetic acid. The time of analysis does not exceed 6 min., the limits of detection vary from 0.1 to 1.7 µmol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal and amniotic fluid samples (en)
Title	2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection 2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection (en)
skos:prefLabel	2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection 2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection (en)
skos:notation	RIV/00216208:11120/12:43906525!RIV13-MSM-11120___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP206/10/1231)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Tůma, Petr
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	183314
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/12:43906525
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Short separation pathway; Clinical samples; Contactless conductivity detection; Capillary electrophoresis; Amino acids (en)
http://linked.open.../riv/klicoveSlovo	Clinical samples Short separation pathway Capillary electrophoresis Amino acids Contactless conductivity detection
http://linked.open...ontrolniKodProRIV	[750BBA97A8C2]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology; Vol. 919
http://linked.open...i/riv/nazevZdroje	Clinical Applications of Capillary Electrophoresis: Methods and Protocols
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...v/pocetStranKnihy	311 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	New Electrophoretic Approaches to the Monitoring of Pain Neurotransmitters in the Brain Tissue of Higher Organisms
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Tůma, Petr
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-029-8_2
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-028-1
http://localhost/t...ganizacniJednotka	11120

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