About: A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://ar.iiarjournals.org/content/31/10/3125.full
Description	Circulating tumor cells (CTCs) are of great importance for cancer diagnosis, prognosis and treatment. It is necessary to improve the ability to image and analyze them for their biological properties which determine their behavior in the patient. In the present study, using immunomagnetic beads, CTCs were rapidly isolated from the circulation of mice orthotopically implanted with human PC-3 prostate cancer cells stably expressing green fluorescent protein (GFP). The PC-3 GFP CTCs were then expanded in culture in parallel with the parental PC-3 GFP cell line. Both cell types were then inoculated onto the chorioallentoic membrane (CAM) of chick embryos. Eight days later, embryos were harvested and the brains were processed for frozen sections. The IV-100 intravital laser scanning microscope enabled rapid identification of fluorescent metastatic foci within the chick embryonic brain. Inoculation of embryos with PC-3 GFP CTCs resulted in a 3 to 10-fold increase in brain metastasis when compared to those wi Circulating tumor cells (CTCs) are of great importance for cancer diagnosis, prognosis and treatment. It is necessary to improve the ability to image and analyze them for their biological properties which determine their behavior in the patient. In the present study, using immunomagnetic beads, CTCs were rapidly isolated from the circulation of mice orthotopically implanted with human PC-3 prostate cancer cells stably expressing green fluorescent protein (GFP). The PC-3 GFP CTCs were then expanded in culture in parallel with the parental PC-3 GFP cell line. Both cell types were then inoculated onto the chorioallentoic membrane (CAM) of chick embryos. Eight days later, embryos were harvested and the brains were processed for frozen sections. The IV-100 intravital laser scanning microscope enabled rapid identification of fluorescent metastatic foci within the chick embryonic brain. Inoculation of embryos with PC-3 GFP CTCs resulted in a 3 to 10-fold increase in brain metastasis when compared to those wi (en)
Title	A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells (en)
skos:prefLabel	A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells A Rapid Imageable In Vivo Metastasis Assay for Circulating Tumor Cells (en)
skos:notation	RIV/00216208:11120/11:00003424!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	N
http://linked.open...avai/riv/aktivity	N
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Bobek, Vladimír Kološtová, Katarína
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	184100
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/11:00003424
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Circulating tumor cells; CTCs; prostate cancer; immunomagnetic beads; GFP; chick embryo; brain metastasis; metastatic assay; fluorescence imaging (en)
http://linked.open.../riv/klicoveSlovo	GFP immunomagnetic beads prostate cancer CTCs Circulating tumor cells brain metastasis chick embryo fluorescence imaging metastatic assay
http://linked.open...odStatuVydavatele	GR - Řecká republika
http://linked.open...ontrolniKodProRIV	[A38EE48350A5]
http://linked.open...i/riv/nazevZdroje	Anticancer Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Bobek, Vladimír Kološtová, Katarína Menen, R. S.
http://linked.open...ain/vavai/riv/wos	000295667700002
issn	0250-7005
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	11120

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