About: The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance

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About: The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A capillary electrophoretic procedure employing capacitively coupled contactless conductivity detection (C4D) has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min., respectively, when using the optimized background electrolyte, 60 mM H3BO3 + 30 mM LiOH (pH 9.1). The limits of detection amount to 0.5 uM for glycerol and 0.3 uM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 % and 3.2 %, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further A capillary electrophoretic procedure employing capacitively coupled contactless conductivity detection (C4D) has been developed for direct determination of the glycerol and mannitol polyalcohols in biological and pharmacological samples. Both glycerol and mannitol are fully separated from the sample matrix within very short times of 3.0 and 3.9 min., respectively, when using the optimized background electrolyte, 60 mM H3BO3 + 30 mM LiOH (pH 9.1). The limits of detection amount to 0.5 uM for glycerol and 0.3 uM for mannitol. The repeatability of the glycerol determination in real biological materials is characterized by the coefficient of variation values, 0.5 % and 3.2 %, for the migration time and the peak area, respectively. The procedure has been used to monitor the free glycerol concentration in adipose tissue microdialyzates. A physiological study has demonstrated that the lipolysis occurring during a sporting action can be stimulated by local application of adrenaline. The procedure has further (en)
Title	The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance (en)
skos:prefLabel	The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance The Use of Capillary Electrophoresis with Contactless Conductivity Detection for Monitoring of Glycerol in Adipose Tissues During a Sporting Performance (en)
skos:notation	RIV/00216208:11120/10:00002405!RIV11-GA0-11120___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA203/07/0896), P(GAP206/10/1231), S, Z(MSM0021620814)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Wedellová, Zuzana Samcová, Eva Tůma, Petr Málková, Klára
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	294641
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/10:00002405
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Adipose tissue; Contactless conductivity detection; Glycerol; Mannitol. (en)
http://linked.open.../riv/klicoveSlovo	Glycerol Adipose tissue Contactless conductivity detection Mannitol.
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[FD67D3DD2EBD]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	New Electrophoretic Approaches to the Monitoring of Pain Neurotransmitters in the Brain Tissue of Higher Organisms Fast screening of biologically active low-molecular weight compounds in biological materials by microfluidic techniques
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Samcová, Eva Tůma, Petr Wedellová, Zuzana Málková, Klára
http://linked.open...ain/vavai/riv/wos	000280057600013
http://linked.open...n/vavai/riv/zamer	Prevention, diagnostics and therapy of diabetes mellitus, metabolic and endocrine damage of organism
issn	0173-0835
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	11120

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