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rdf:type
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Description
| - Employing proteomic techniques (isolation of membrane proteins, protein separation by high-resolution 2D electrophoresis, computer differential analysis, and tandem mass spectrometry) we identified two proteins with statistically significant upregulation under iron deprivation in membrane fraction of K562 cells (human erythroleukemia), i.e., aldolase A (ALDA) and voltage-dependent anion channel 2 (VDAC2). The increased expression of aldolase A and VDAC2 under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated.
- Employing proteomic techniques (isolation of membrane proteins, protein separation by high-resolution 2D electrophoresis, computer differential analysis, and tandem mass spectrometry) we identified two proteins with statistically significant upregulation under iron deprivation in membrane fraction of K562 cells (human erythroleukemia), i.e., aldolase A (ALDA) and voltage-dependent anion channel 2 (VDAC2). The increased expression of aldolase A and VDAC2 under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated. (en)
- Použitím proteomických technik (izolace membránových proteinů, separace proteinů pomocí 2D elektroforézy s vysokou rozlišovací schopností, počítačová diferenciální analýza a tandemová hmotnostní spektrometrie) jsme identifikovali dva proteiny se statisticky významnou upregulací při deprivaci železa v membránové frakci buněk K562 (lidská erytroleukémie): aldolázu A (ALDA) a voltage-dependent anion channel 2 (VDAC2). Zvýšená exprese aldolázy A a VDAC2 při deprivaci železa byla také potvrzena pomocí western blot analýzy. Poprvé jsme demonstrovali, že exprese aldolázy A a VDAC2 může být kontrolována hladinou železa. (cs)
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Title
| - VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation
- VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí při deprivaci železa (cs)
- VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation (en)
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skos:prefLabel
| - VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation
- VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí při deprivaci železa (cs)
- VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation (en)
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skos:notation
| - RIV/00216208:11120/08:00000522!RIV09-AV0-11120___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(IAA5052402), Z(AV0Z50200510), Z(AV0Z50520701), Z(MSM0021620814)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11120/08:00000522
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Iron deprivation; K562 cells; Membrane proteins; Aldolase A; VDAC2 (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Molecular and Cellular Biochemistry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kovář, Jan
- Neubauerová, Jitka
- Man, P.
- Vališ, K.
- Pompach, P.
- Vohradský, J.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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