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| rdf:type
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| Description
| - mmunolabeling of isolated plasma membrane (PM) sheets combined with high-resolution electron microscopy is a powerful technique for understanding the topography of PM-bound signaling molecules. However, this technique has been mostly confined to analysis of membrane sheets from adherent cells. Here we present a rapid, simple and versatile method for isolation of PM sheets from non-adherent cells, and show its use for examination of the topography of Fcɛ receptor I (FcɛRI) and transmembrane adaptors, LAT (linker for activation of T cells) and NTAL (non-T cell activation linker), in murine bone marrow-derived mast cells (BMMC). The data were compared with those obtained from widely used but tumor-derived rat basophilic leukemia (RBL) cells. In non-activated cells, FcɛRI was distributed either individually or in small clusters of comparable size in both cell types. In multivalent antigen-activated BMMC as well as RBL cells, FcɛRI was internalized to a similar extent, b
- mmunolabeling of isolated plasma membrane (PM) sheets combined with high-resolution electron microscopy is a powerful technique for understanding the topography of PM-bound signaling molecules. However, this technique has been mostly confined to analysis of membrane sheets from adherent cells. Here we present a rapid, simple and versatile method for isolation of PM sheets from non-adherent cells, and show its use for examination of the topography of Fcɛ receptor I (FcɛRI) and transmembrane adaptors, LAT (linker for activation of T cells) and NTAL (non-T cell activation linker), in murine bone marrow-derived mast cells (BMMC). The data were compared with those obtained from widely used but tumor-derived rat basophilic leukemia (RBL) cells. In non-activated cells, FcɛRI was distributed either individually or in small clusters of comparable size in both cell types. In multivalent antigen-activated BMMC as well as RBL cells, FcɛRI was internalized to a similar extent, b (en)
- V práci jsou popsány nové vlastnosti signálních komplexů vysokoafinitního IgE receptoru na žírných buňkách. Výsledky jsou prezentovány pomocí nově vyvinuté metody na izolaci membrán z neadherentních buněk imunitního systému, která je v práci podrobně popsána a její účinnost vyhodnocena (cs)
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| Title
| - Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells
- Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells (en)
- Topografie signálních molekul detekovaných pomocí elektronové mikroskopie na plasmatických membránách izolovaných z neadherentních žírných buněk (cs)
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| skos:prefLabel
| - Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells
- Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells (en)
- Topografie signálních molekul detekovaných pomocí elektronové mikroskopie na plasmatických membránách izolovaných z neadherentních žírných buněk (cs)
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| skos:notation
| - RIV/00216208:11120/07:00000230!RIV08-GA0-11120___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1M0506), P(GA301/06/0361), P(GD204/05/H023), Z(AV0Z50520514), Z(MSM0021620814)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11120/07:00000230
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - mast cell; high affinity IgE receptor; plasma membrane sheets isolation; electron microscopy (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Immunological Methods
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Heneberg, Petr
- Dráber, P.
- Korb, J.
- Lebduška, P.
- Tůmová, M.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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