About: Calprotectin expression and mononuclear phagocyte subpopulations in peripheral blood and bronchoalveolar lavage     Goto   Sponge   NotDistinct   Permalink

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  • The phenotype of human alveolar macrophages (AM) can be affected by the process of maturation/ differentiation and by multiple factors from the local environment. The aim of our study was to assess the expression of selected phenotypic markers characteristic for subsets of mononuclear phagocytes in bronchoalveolar lavage fluid (BAL) and peripheral blood with special attention to calprotectin (27E10), a marker of acute inflammatory macrophages. The expression of calprotectin and 13 other phenotypic markers was evaluated by an immunoperoxidase slide assay and computer image analysis. We consider calprotectin (27E10 antigen) to be a marker of freshly recruited, monocyte-like, mononuclear phagocytes. being expressed in 84 +/- 13% PBM and only 10 +/- 11% Cre of AM, p < 0.001. Computer image analysis confirmed that calprotectin-positive mononuclear cells in peripheral blood and BAL are morphologically very similar in contrast to the much larger calprotectin-negative AM. On the other hand, 25F9 antigen, t
  • The phenotype of human alveolar macrophages (AM) can be affected by the process of maturation/ differentiation and by multiple factors from the local environment. The aim of our study was to assess the expression of selected phenotypic markers characteristic for subsets of mononuclear phagocytes in bronchoalveolar lavage fluid (BAL) and peripheral blood with special attention to calprotectin (27E10), a marker of acute inflammatory macrophages. The expression of calprotectin and 13 other phenotypic markers was evaluated by an immunoperoxidase slide assay and computer image analysis. We consider calprotectin (27E10 antigen) to be a marker of freshly recruited, monocyte-like, mononuclear phagocytes. being expressed in 84 +/- 13% PBM and only 10 +/- 11% Cre of AM, p < 0.001. Computer image analysis confirmed that calprotectin-positive mononuclear cells in peripheral blood and BAL are morphologically very similar in contrast to the much larger calprotectin-negative AM. On the other hand, 25F9 antigen, t (en)
Title
  • Calprotectin expression and mononuclear phagocyte subpopulations in peripheral blood and bronchoalveolar lavage
  • Calprotectin expression and mononuclear phagocyte subpopulations in peripheral blood and bronchoalveolar lavage (en)
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  • Calprotectin expression and mononuclear phagocyte subpopulations in peripheral blood and bronchoalveolar lavage
  • Calprotectin expression and mononuclear phagocyte subpopulations in peripheral blood and bronchoalveolar lavage (en)
skos:notation
  • RIV/00216208:11120/01:00003808!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita
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  • Z(MSM 111200002)
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  • 1
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  • 674862
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  • RIV/00216208:11120/01:00003808
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  • macrophages; phenotype; calprotectin; bronchoalveolar lavage (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IT - Italská republika
http://linked.open...ontrolniKodProRIV
  • [D342DA81EA7E]
http://linked.open...i/riv/nazevZdroje
  • Sarcoidosis Vasculitis and Diffuse Lung Diseases
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 18
http://linked.open...iv/tvurceVysledku
  • Stříž, Ilja
http://linked.open...ain/vavai/riv/wos
  • 000167930600007
http://linked.open...n/vavai/riv/zamer
issn
  • 1124-0490
number of pages
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  • 11120
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