About: Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio Nuclear speckles (speckles) represent a distinct nuclear compartment within the interchromatin space and are enriched in splicing factors. They have been shown to serve neighboring active genes as a reservoir of these factors. In this study, we show that, in HeLa cells, the (pre)spliceosomal assembly on precursor mRNA (pre-mRNA) is associated with the speckles. For this purpose, we used microinjection of splicing competent and mutant adenovirus pre-mRNAs with differential splicing factor binding, which form different (pre)spliceosomal complexes and followed their sites of accumulation. Splicing competent pre-mRNAs are rapidly targeted into the speckles, but the targeting is temperature-dependent. The polypyrimidine tract sequence is required for targeting, but, in itself, is not sufficient. The downstream flanking sequences are particularly important for the targeting of the mutant pre-mRNAs into the speckles. In supportive experiments, the behavior of the speckles was followed after the microinjectio (en)
Title	Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles (en)
skos:prefLabel	Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles (en)
skos:notation	RIV/00216208:11120/01:00003804!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(MSM 111100003)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kopský, Vojtěch Raška, Ivan Melčák, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	692605
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/01:00003804
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Pre-Messenger-Rna; Polymerase-Ii Transcription; Ribonucleoprotein-Particles; Splicing Factors; Living Cells; Nascent Transcripts; Functional U1; Sc-35 Domains; Sr Proteins; Localization (en)
http://linked.open.../riv/klicoveSlovo	Functional U1 Living Cells Polymerase-Ii Transcription Pre-Messenger-Rna Ribonucleoprotein-Particles Sc-35 Domains Splicing Factors Sr Proteins Localization Nascent Transcripts
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1DB13147DA05]
http://linked.open...i/riv/nazevZdroje	Molecular Biology of the Cell
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional organization of the cell nucleus Dynamic structure and function in the cell nucleus Laboratory of gene expression
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Raška, Ivan Kopský, Vojtěch Melčák, Ivo
http://linked.open...ain/vavai/riv/wos	000170348000012
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20111100003
issn	1059-1524
number of pages	14 (xsd:int)
http://localhost/t...ganizacniJednotka	11120

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