About: The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase

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About: The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside S-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2'-deoxythymidine analogues 5-chloro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2'-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase. Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside S-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2'-deoxythymidine analogues 5-chloro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2'-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase. (en)
Title	The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase (en)
skos:prefLabel	The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase (en)
skos:notation	RIV/00216208:11120/01:00003803!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA302/99/0587), P(GA304/00/1481), P(VS96129), Z(AV0Z4055905), Z(AV0Z5039906)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Raška, Ivan Staněk, David Malinský, Jan Koberna, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	697901
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/01:00003803
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	replication; speed of replication fork; deoxynucleotide; chlorodeoxyuridine; iododeoxyuridine (en)
http://linked.open.../riv/klicoveSlovo	replication chlorodeoxyuridine iododeoxyuridine speed of replication fork deoxynucleotide
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[D341AB9CF08C]
http://linked.open...i/riv/nazevZdroje	Journal of Cell Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional organization of the cell nucleus Dynamic structure and function in the cell nucleus Laboratory of gene expression
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	114
http://linked.open...iv/tvurceVysledku	Raška, Ivan Staněk, David Malínský, Jan Koberna, Karel
http://linked.open...ain/vavai/riv/wos	000167441400014
http://linked.open...n/vavai/riv/zamer	The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy Chemical principles of selected biological phenomena in medicine and ecology
issn	0021-9533
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	11120

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