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rdf:type
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rdfs:seeAlso
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Description
| - The study was aimed at determining the genotoxic potential of extractable organic matter (EOM) from ambient air particles PM10 (< 10 mu m) using mammalian cells in culture as test system. Air samples were collected in the course of summer and winter periods in two regions of the Czech Republic representing low and high levels of air pollution, the districts of industrial Teplice and rural Prachatice, respectively. EOM was fractionated by acid-base partitioning and silica gel column chromatography. Aliquots of fractions were incubated with cultured hepatocytes derived from male rats or Chinese hamster lung V79NH cells expressing nitroreductase activity but virtually no cytochrome P450 activity. DNA adduct levels were analyzed by P-32-postlabeling using butanol extraction for adduct enrichment. In hepatocytes, crude extracts caused the formation of substantial amounts of DNA reactive material bring detectable in a broad diagonal radioactive zone (DRZ) in the chromatograms. Highest DNA adduct levels w
- The study was aimed at determining the genotoxic potential of extractable organic matter (EOM) from ambient air particles PM10 (< 10 mu m) using mammalian cells in culture as test system. Air samples were collected in the course of summer and winter periods in two regions of the Czech Republic representing low and high levels of air pollution, the districts of industrial Teplice and rural Prachatice, respectively. EOM was fractionated by acid-base partitioning and silica gel column chromatography. Aliquots of fractions were incubated with cultured hepatocytes derived from male rats or Chinese hamster lung V79NH cells expressing nitroreductase activity but virtually no cytochrome P450 activity. DNA adduct levels were analyzed by P-32-postlabeling using butanol extraction for adduct enrichment. In hepatocytes, crude extracts caused the formation of substantial amounts of DNA reactive material bring detectable in a broad diagonal radioactive zone (DRZ) in the chromatograms. Highest DNA adduct levels w (en)
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Title
| - Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter
- Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter (en)
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skos:prefLabel
| - Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter
- Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter (en)
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skos:notation
| - RIV/00216208:11120/00:00003732!RIV12-MSM-11120___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - N, P(SI/340/2/00), Z(AV0Z5039906)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11120/00:00003732
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - genotoxicity; DNA adducts; P-32-postlabeling; hepatocytes; V79NH cells (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Černá, Milena
- Topinka, J.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1016/S1383-5718(00)00061-9
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http://localhost/t...ganizacniJednotka
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