About: Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter

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About: Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/S1383-5718(00)00061-9
Description	The study was aimed at determining the genotoxic potential of extractable organic matter (EOM) from ambient air particles PM10 (< 10 mu m) using mammalian cells in culture as test system. Air samples were collected in the course of summer and winter periods in two regions of the Czech Republic representing low and high levels of air pollution, the districts of industrial Teplice and rural Prachatice, respectively. EOM was fractionated by acid-base partitioning and silica gel column chromatography. Aliquots of fractions were incubated with cultured hepatocytes derived from male rats or Chinese hamster lung V79NH cells expressing nitroreductase activity but virtually no cytochrome P450 activity. DNA adduct levels were analyzed by P-32-postlabeling using butanol extraction for adduct enrichment. In hepatocytes, crude extracts caused the formation of substantial amounts of DNA reactive material bring detectable in a broad diagonal radioactive zone (DRZ) in the chromatograms. Highest DNA adduct levels w The study was aimed at determining the genotoxic potential of extractable organic matter (EOM) from ambient air particles PM10 (< 10 mu m) using mammalian cells in culture as test system. Air samples were collected in the course of summer and winter periods in two regions of the Czech Republic representing low and high levels of air pollution, the districts of industrial Teplice and rural Prachatice, respectively. EOM was fractionated by acid-base partitioning and silica gel column chromatography. Aliquots of fractions were incubated with cultured hepatocytes derived from male rats or Chinese hamster lung V79NH cells expressing nitroreductase activity but virtually no cytochrome P450 activity. DNA adduct levels were analyzed by P-32-postlabeling using butanol extraction for adduct enrichment. In hepatocytes, crude extracts caused the formation of substantial amounts of DNA reactive material bring detectable in a broad diagonal radioactive zone (DRZ) in the chromatograms. Highest DNA adduct levels w (en)
Title	Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter (en)
skos:prefLabel	Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter Genotoxicity of urban air pollutants in the Czech Republic Part II. DNA adduct formation in mammalian cells by extractable organic matter (en)
skos:notation	RIV/00216208:11120/00:00003732!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	N P Z
http://linked.open...avai/riv/aktivity	N, P(SI/340/2/00), Z(AV0Z5039906)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Černá, Milena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	711907
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/00:00003732
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	genotoxicity; DNA adducts; P-32-postlabeling; hepatocytes; V79NH cells (en)
http://linked.open.../riv/klicoveSlovo	P-32-postlabeling DNA adducts genotoxicity hepatocytes V79NH cells
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[F6CC9CD5AD0B]
http://linked.open...i/riv/nazevZdroje	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
http://linked.open...in/vavai/riv/obor	DN
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Impact of complex mixtures in polluted air on the health of risk population groups( Program Air pollution and health)
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	469
http://linked.open...iv/tvurceVysledku	Černá, Milena Topinka, J.
http://linked.open...ain/vavai/riv/wos	000089091300009
http://linked.open...n/vavai/riv/zamer	The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy
issn	1383-5718
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/S1383-5718(00)00061-9
http://localhost/t...ganizacniJednotka	11120

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