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| Description
| - Background InformationThe optimal repair of DNA lesions is fundamental for physiological processes. We asked whether the recruitment of HP1, 53BP1 and BMI1 proteins to ultraviolet (UVA)-induced DNA lesions requires functional A-type lamins. ResultsWe found that UVA irradiation of nuclear lamina abolished the fluorescence of mCherry-tagged A-type lamins and destroyed the nuclear lamina as also observed by electron microscopy studies. Similarly, an absence of endogenous A- and B-type lamins was found in irradiated regions by UVA. However, irradiation did not affect the recruitment of HP1, 53BP1 and BMI1 to DNA lesions. The UVA-induced shrinkage of the nuclear lamina, which anchors chromatin, explains why UVA-micro-irradiated chromatin is relaxed. Conversely, additional experiments with -irradiation showed that the nuclear lamina remained intact and the genome-wide level of HP1 was stable. Fluorescence intensity of HP1 and BMI1 in UVA-induced DNA lesions and level of HP1 after -irradiation were unaffected by deficiency in A-type lamins, whereas those parameters of 53BP1 were changed. ConclusionsWe conclude that only the 53BP1 status in DNA lesions, induced by UVA or -rays, is affected by A-type lamin deficiency, which was not observed for heterochromatin-related proteins HP1 and BMI1.
- Background InformationThe optimal repair of DNA lesions is fundamental for physiological processes. We asked whether the recruitment of HP1, 53BP1 and BMI1 proteins to ultraviolet (UVA)-induced DNA lesions requires functional A-type lamins. ResultsWe found that UVA irradiation of nuclear lamina abolished the fluorescence of mCherry-tagged A-type lamins and destroyed the nuclear lamina as also observed by electron microscopy studies. Similarly, an absence of endogenous A- and B-type lamins was found in irradiated regions by UVA. However, irradiation did not affect the recruitment of HP1, 53BP1 and BMI1 to DNA lesions. The UVA-induced shrinkage of the nuclear lamina, which anchors chromatin, explains why UVA-micro-irradiated chromatin is relaxed. Conversely, additional experiments with -irradiation showed that the nuclear lamina remained intact and the genome-wide level of HP1 was stable. Fluorescence intensity of HP1 and BMI1 in UVA-induced DNA lesions and level of HP1 after -irradiation were unaffected by deficiency in A-type lamins, whereas those parameters of 53BP1 were changed. ConclusionsWe conclude that only the 53BP1 status in DNA lesions, induced by UVA or -rays, is affected by A-type lamin deficiency, which was not observed for heterochromatin-related proteins HP1 and BMI1. (en)
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| Title
| - Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins
- Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins (en)
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| skos:prefLabel
| - Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins
- Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins (en)
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| skos:notation
| - RIV/00216208:11110/14:10285499!RIV15-MSM-11110___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - I, P(ED1.1.00/02.0109), P(GA13-07822S), P(GAP302/10/1022), P(GBP302/12/G157)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11110/14:10285499
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Protein mobility; Lamins; HP1; DNA damage; Chromatin (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bártová, Eva
- Kozubek, Stanislav
- Legartová, Soňa
- Cmarko, Dušan
- Sehnalová, Petra
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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