About: Tracking DNA and RNA Sequences at High Resolution

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/protocol/10.1007%2F978-1-62703-776-1_16
Description	In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol for the post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail. In this chapter, the basic principles and protocols of the electron microscopical detections of specific DNA and RNA sequences are described. We focused primarily on a comparison of various methods of electron microscopy in situ hybridization (EM-ISH) with respect to their sensitivity and the structural preservation of the sample with the aim of helping the readers select the appropriate hybridization protocol. As the post-embedding EM-ISH most frequently represents the optimal choice, the protocol for the post-embedding EM-ISH approach is described in detail. Concurrently, the alternative methods based on the enzymatic synthesis of the labeled nucleic acids chains that can be used for the detection of DNA or RNA molecules in situ are mentioned. In this respect, the technique enabling the enzymatic detection of the polyadenylated RNA sequences is described in detail. (en)
Title	Tracking DNA and RNA Sequences at High Resolution Tracking DNA and RNA Sequences at High Resolution (en)
skos:prefLabel	Tracking DNA and RNA Sequences at High Resolution Tracking DNA and RNA Sequences at High Resolution (en)
skos:notation	RIV/00216208:11110/14:10284235!RIV15-MSM-11110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0030/01/01), P(ED1.1.00/02.0109), P(GAP302/12/1885), P(GBP302/12/G157), P(TA03010598), P(TA03010719)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Cmarko, Dušan
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	50894
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/14:10284235
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Freeze substitution; High-pressure freezing; Cryo-sections; Pre-embedding; Post-embedding; In situ hybridization; Double- and single-stranded DNA and RNA; Electron microscopy (en)
http://linked.open.../riv/klicoveSlovo	Electron microscopy In situ hybridization Cryo-sections Double- and single-stranded DNA and RNA Freeze substitution Post-embedding Pre-embedding High-pressure freezing
http://linked.open...ontrolniKodProRIV	[85E191CA1F00]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology, 1117
http://linked.open...i/riv/nazevZdroje	Electron Microscopy; Methods and Protocols : Methods and Protocols
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...v/pocetStranKnihy	799 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University Biomedicine for regional development and human resources Relationship between replication of ribosomal genes and their transcription Modified nucleosides, nucleotides and oligonucleotides and their application in biomedicine Novel Tools for Cell Cycle Analysis Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Cmarko, Dušan Koberna, Karel Ligasová, Anna
number of pages	24 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-776-1_16
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-775-4
http://localhost/t...ganizacniJednotka	11110

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