About: Microscopic analysis of chromatin localization and dynamics in C. elegans

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/protocol/10.1007%2F978-1-62703-526-2_11#
Description	During development, the genome undergoes drastic reorganization within the nuclear space. To determine tridimensional genome folding, genome-wide techniques (damID/Hi-C) can be applied using cell populations, but these have to be calibrated using microscopy and single-cell analysis of gene positioning. Moreover, the dynamic behavior of chromatin has to be assessed on living samples. Combining fast stereotypic development with easy genetics and microscopy, the nematode C. elegans has become a model of choice in recent years to study changes in nuclear organization during cell fate acquisition. Here we present two complementary techniques to evaluate nuclear positioning of genes either by fluorescence in situ hybridization in fixed samples or in living worm embryos using the GFP-lacI/lacO chromatin-tagging system. During development, the genome undergoes drastic reorganization within the nuclear space. To determine tridimensional genome folding, genome-wide techniques (damID/Hi-C) can be applied using cell populations, but these have to be calibrated using microscopy and single-cell analysis of gene positioning. Moreover, the dynamic behavior of chromatin has to be assessed on living samples. Combining fast stereotypic development with easy genetics and microscopy, the nematode C. elegans has become a model of choice in recent years to study changes in nuclear organization during cell fate acquisition. Here we present two complementary techniques to evaluate nuclear positioning of genes either by fluorescence in situ hybridization in fixed samples or in living worm embryos using the GFP-lacI/lacO chromatin-tagging system. (en)
Title	Microscopic analysis of chromatin localization and dynamics in C. elegans Microscopic analysis of chromatin localization and dynamics in C. elegans (en)
skos:prefLabel	Microscopic analysis of chromatin localization and dynamics in C. elegans Microscopic analysis of chromatin localization and dynamics in C. elegans (en)
skos:notation	RIV/00216208:11110/13:10194761!RIV14-GA0-11110___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP302/11/1262), P(GBP302/12/G157)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Lanctôt, Christian
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	88211
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/13:10194761
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Microscopy; GFP-lacI/lacO; Chromatin tagging; Fluorescence in situ hybridization; C. elegans; Nuclear organization (en)
http://linked.open.../riv/klicoveSlovo	Microscopy Chromatin tagging GFP-lacI/lacO Nuclear organization Fluorescence in situ hybridization C. elegans
http://linked.open...ontrolniKodProRIV	[83FA3185FBC7]
http://linked.open...i/riv/mistoVydani	New York, NY, USA
http://linked.open...vEdiceCisloSvazku	1042
http://linked.open...i/riv/nazevZdroje	Imaging Gene Expression
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...v/pocetStranKnihy	368 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions Morphogenesis of the genome during early embryonic development of C. elegans
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Lanctôt, Christian Meister, Peter
number of pages	20 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-526-2_11
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-525-5
http://localhost/t...ganizacniJednotka	11110

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