About: Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/s12223-012-0181-y
Description	Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus. Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus. (en)
Title	Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus (en)
skos:prefLabel	Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus (en)
skos:notation	RIV/00216208:11110/13:10189736!RIV14-GA0-11110___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA310/07/0675), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Nováková, Michaela Čechová, Dana Zanvit, Peter Prokešová, Ludmila Novotná, Olga Julák, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	78915
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/13:10189736
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokines; infection; vaccine; mucosal; adjuvant; in-vitro; immune-response; intranasal immunization; influenza-virus; toxin-b subunit (en)
http://linked.open.../riv/klicoveSlovo	adjuvant infection influenza-virus intranasal immunization mucosal toxin-b subunit cytokines immune-response vaccine in-vitro
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[82111ABD8746]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Mucosal immunization by influenza virus type A
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Julák, Jaroslav Prokešová, Ludmila Zanvit, Peter Novotná, Olga Nováková, Michaela Mikulik, Karel Čechová, Dana
http://linked.open...ain/vavai/riv/wos	000314409400003
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	0015-5632
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12223-012-0181-y
http://localhost/t...ganizacniJednotka	11110

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