About: Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
rdfs:seeAlso
Description
  • Magnetic bead cellulose activated with divinyl sulfone was used for the immobilization of Staphylococcus aureus endoproteinase Glu-C (EC 3.4.21.19). The immobilized proteinase was characterized by increased thermostability, by decreased self-cleavage activity, and a possibility of repeated use. The prepared immobilized enzyme was applied for the proteolytic cleavage of -casein and BSA under different conditions (different composition of buffers, different pH, and different time of digestion). The possibilities of the direct use of enzyme reaction products for MALDI TOF MS analysis were shown.
  • Magnetic bead cellulose activated with divinyl sulfone was used for the immobilization of Staphylococcus aureus endoproteinase Glu-C (EC 3.4.21.19). The immobilized proteinase was characterized by increased thermostability, by decreased self-cleavage activity, and a possibility of repeated use. The prepared immobilized enzyme was applied for the proteolytic cleavage of -casein and BSA under different conditions (different composition of buffers, different pH, and different time of digestion). The possibilities of the direct use of enzyme reaction products for MALDI TOF MS analysis were shown. (en)
Title
  • Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis
  • Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis (en)
skos:prefLabel
  • Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis
  • Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis (en)
skos:notation
  • RIV/00216208:11110/13:10189373!RIV14-GA0-11110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GAP206/12/0381)
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 78899
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11110/13:10189373
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Magnetic bead cellulose; Immobilized endoproteinase; Glu-C; Enzyme immobilization; -Casein; BSA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [B88DBE9DE071]
http://linked.open...i/riv/nazevZdroje
  • Journal of Separation Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 36
http://linked.open...iv/tvurceVysledku
  • Tichá, Marie
  • Kučerová, Zdenka
  • Přikryl, Petr
http://linked.open...ain/vavai/riv/wos
  • 000320553800023
issn
  • 1615-9306
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jssc.201300118
http://localhost/t...ganizacniJednotka
  • 11110
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software