About: Mannitol is not involved in protective reactions of Acanthamoeba

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About: Mannitol is not involved in protective reactions of Acanthamoeba Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.molbiopara.2012.04.010
Description	Genes for mannitol-metabolizing enzymes, mannitol phosphate dehydrogenase (MPDH) and mannitol dehydrogenase (MDH), have been recently identified in the genome of Acanthamoeba castellanii and their potential role in stress tolerance was proposed. Using qRT-PCR, comparison has been made of mRNA levels of the enzymes for mannitol metabolism at various time intervals during the stress defence reactions of encystation and pseudocyst formation. Gradual decrease of both enzymes during encystation and slight increases at the beginning of pseudocyst formation were observed. Detailed analysis of mRNA sequences of the two genes revealed similarities with various alcohol dehydrogenases rather than mannitol dehydrogenases. Our results indicate there is probably no protective role for mannitol in Acanthamoeba as no mannitol was detected using HILIC ESI MS, in any Acanthamoeba life cycle stage. Possible misinterpretation of previously published sequences as encoding enzymes of the mannitol metabolic pathway is discussed. Genes for mannitol-metabolizing enzymes, mannitol phosphate dehydrogenase (MPDH) and mannitol dehydrogenase (MDH), have been recently identified in the genome of Acanthamoeba castellanii and their potential role in stress tolerance was proposed. Using qRT-PCR, comparison has been made of mRNA levels of the enzymes for mannitol metabolism at various time intervals during the stress defence reactions of encystation and pseudocyst formation. Gradual decrease of both enzymes during encystation and slight increases at the beginning of pseudocyst formation were observed. Detailed analysis of mRNA sequences of the two genes revealed similarities with various alcohol dehydrogenases rather than mannitol dehydrogenases. Our results indicate there is probably no protective role for mannitol in Acanthamoeba as no mannitol was detected using HILIC ESI MS, in any Acanthamoeba life cycle stage. Possible misinterpretation of previously published sequences as encoding enzymes of the mannitol metabolic pathway is discussed. (en)
Title	Mannitol is not involved in protective reactions of Acanthamoeba Mannitol is not involved in protective reactions of Acanthamoeba (en)
skos:prefLabel	Mannitol is not involved in protective reactions of Acanthamoeba Mannitol is not involved in protective reactions of Acanthamoeba (en)
skos:notation	RIV/00216208:11110/12:11363!RIV13-MSM-11110___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA310/09/1120), S, Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Nohýnková, Eva Klieščiková, Jarmila Bínová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	148051
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/12:11363
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Acanthamoeba; mannitol; mannitol dehydrogenase; mannitol phosphate dehydrogenase; encystation; cyst; pseudocyst (en)
http://linked.open.../riv/klicoveSlovo	cyst encystation mannitol mannitol dehydrogenase mannitol phosphate dehydrogenase Acanthamoeba pseudocyst
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[06429D0BBCFD]
http://linked.open...i/riv/nazevZdroje	Molecular and Biochemical Parasitology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Differentiation of free living amoebae of genera Acanthamoeba and Balamuthia
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	184
http://linked.open...iv/tvurceVysledku	Nohýnková, Eva Klieščiková, Jarmila Bínová, Eva Ashford, David A. Thomas-Oates, Jane
http://linked.open...ain/vavai/riv/wos	000306626300007
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	0166-6851
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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