About: Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction

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About: Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A comparison of histochemical detection of GM1 ganglioside in cryostat sections using cholera toxin B-subunit after fixation with 4% formaldehyde and dry acetone gave tissue-dependant results. In the liver neither pretreatment showed detectable differences relative to GM1 reaction products, while studies in the brain showed the superiority of acetone preextraction (followed by formaldehyde), which yielded sharper images compared to the diffuse, blurred staining pattern associated with formaldehyde. Thus, anhydrous conditions (dry tissue samples and anhydrous acetone) are crucial factors for optimal in situ ganglioside detection using acetone pretreatment. This insures effective physical fixation (precipitation, prevention of in situ diffusion),, especially in tissues rich in polar lipids and removal of cholesterol, which can act as a hydrophobic blocking barrier. A comparison of histochemical detection of GM1 ganglioside in cryostat sections using cholera toxin B-subunit after fixation with 4% formaldehyde and dry acetone gave tissue-dependant results. In the liver neither pretreatment showed detectable differences relative to GM1 reaction products, while studies in the brain showed the superiority of acetone preextraction (followed by formaldehyde), which yielded sharper images compared to the diffuse, blurred staining pattern associated with formaldehyde. Thus, anhydrous conditions (dry tissue samples and anhydrous acetone) are crucial factors for optimal in situ ganglioside detection using acetone pretreatment. This insures effective physical fixation (precipitation, prevention of in situ diffusion),, especially in tissues rich in polar lipids and removal of cholesterol, which can act as a hydrophobic blocking barrier. (en)
Title	Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction (en)
skos:prefLabel	Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction (en)
skos:notation	RIV/00216208:11110/10:7922!RIV11-MZ0-11110___
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(NR9366), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Vítek, Libor Petr, Tomáš Muchová, Lucie Šmíd, Václav Šmídová, Jaroslava Elleder, Milan Hůlková, Helena Šmíd, František Jirkovská, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	261629
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/10:7922
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fixation; GM1 ganglioside; cholera toxin; anhydrous acetone; 4% formaldehyde; central-nervous-system; antiganglioside antibodies; globotriaosyl ceramide; monoclonal-antibody; localization; membrane; binding; glycosphingolipids; microdomains; cholesterol (en)
http://linked.open.../riv/klicoveSlovo	microdomains central-nervous-system cholesterol localization binding membrane 4% formaldehyde anhydrous acetone cholera toxin globotriaosyl ceramide monoclonal-antibody antiganglioside antibodies glycosphingolipids GM1 ganglioside fixation
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[5EA9F0296831]
http://linked.open...i/riv/nazevZdroje	European Journal of Histochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of heme catabolic pathway in in the pathogenesis of cholestasis
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Elleder, Milan Muchová, Lucie Petr, Tomáš Vítek, Libor Hůlková, Helena Šmíd, František Jirkovská, Marie Šmíd, Václav Šmídová, Jaroslava
http://linked.open...ain/vavai/riv/wos	000279051000010
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	1121-760X
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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