About: Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mammalian CcO (cytochrome c oxidase) is a complex composed of 13 different structural subunits encoded by both the mitochondrial and nuclear genomes. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residual enzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdowns and cells overexpressing epitope-tagged Cox6a, Cox7a and Cox7b identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated the interdependence of Cox4 and Cox5a assembly. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells contrary to the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expression programme. Mammalian CcO (cytochrome c oxidase) is a complex composed of 13 different structural subunits encoded by both the mitochondrial and nuclear genomes. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residual enzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdowns and cells overexpressing epitope-tagged Cox6a, Cox7a and Cox7b identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated the interdependence of Cox4 and Cox5a assembly. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells contrary to the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expression programme. (en)
Title	Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b (en)
skos:prefLabel	Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b (en)
skos:notation	RIV/00216208:11110/10:6854!RIV11-GA0-11110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0520), P(GA303/07/0781), P(NS10581), Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	part 3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Stibůrek, Lukáš Vinšová, Kamila Fornůsková, Daniela Wenchich, László Zeman, Jiří Hansíková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	275435
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/10:6854
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytochrome c oxidase; protein assembly; respiratory supercomplex; RNA interference (RNAi) (en)
http://linked.open.../riv/klicoveSlovo	cytochrome c oxidase RNA interference (RNAi) protein assembly respiratory supercomplex
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[96A0339E57FF]
http://linked.open...i/riv/nazevZdroje	Biochemical Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Genetic bases of cytochrome c oxidase deficiency Center for Applied Genomics Biogenesis and genetic defects of cytochrome c oxidase
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	428
http://linked.open...iv/tvurceVysledku	Wenchich, László Zeman, Jiří Hansíková, Hana Vinšová, Kamila Fornůsková, Daniela Stibůrek, Lukáš
http://linked.open...ain/vavai/riv/wos	000278914400005
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	0264-6021
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 77 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software