About: Optimized Protocol for Gene Expression Analysis in Formalin-fixed, Paraffin-embedded Tissue Using Real-time Quantitative Polymerase Chain Reaction

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common tissue specimen available after microscopic examination. However, increasing application of RNA extracted from FFPE tissue frequently results in very small and degraded quantities of nucleic acid. This study targets gene expression analysis from FFPE specimens using real-time quantitative PCR. The whole protocol consists of several steps, that is, RNA extraction and its quality control, reverse transcription, and fluorescence detection during real-time quantitative PCR. We compared several methods in each step, chose the most effective, and with that combination we successfully examined 95% (62 from 65) FFPE samples for our genes of interest. We reached the best results with RNA isolation by using a commercial kit, carefully interpreted UV spectrophotometric values, and meticulously chose reverse transcriptase and TaqMan fluorescence detection. Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common tissue specimen available after microscopic examination. However, increasing application of RNA extracted from FFPE tissue frequently results in very small and degraded quantities of nucleic acid. This study targets gene expression analysis from FFPE specimens using real-time quantitative PCR. The whole protocol consists of several steps, that is, RNA extraction and its quality control, reverse transcription, and fluorescence detection during real-time quantitative PCR. We compared several methods in each step, chose the most effective, and with that combination we successfully examined 95% (62 from 65) FFPE samples for our genes of interest. We reached the best results with RNA isolation by using a commercial kit, carefully interpreted UV spectrophotometric values, and meticulously chose reverse transcriptase and TaqMan fluorescence detection. (en)
Title	Optimized Protocol for Gene Expression Analysis in Formalin-fixed, Paraffin-embedded Tissue Using Real-time Quantitative Polymerase Chain Reaction Optimized Protocol for Gene Expression Analysis in Formalin-fixed, Paraffin-embedded Tissue Using Real-time Quantitative Polymerase Chain Reaction (en)
skos:prefLabel	Optimized Protocol for Gene Expression Analysis in Formalin-fixed, Paraffin-embedded Tissue Using Real-time Quantitative Polymerase Chain Reaction Optimized Protocol for Gene Expression Analysis in Formalin-fixed, Paraffin-embedded Tissue Using Real-time Quantitative Polymerase Chain Reaction (en)
skos:notation	RIV/00216208:11110/09:5491!RIV10-MSM-11110___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620808)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Votavová, Hana Stříteský, Jan Forsterová, Kristina Trněný, Marek Velenská, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	332046
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/09:5491
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	formalin-fixed paraffin-embedded tissue; gene expression; real-time quantitative polymerase chain reaction; reverse transcription; TaqMan probe (en)
http://linked.open.../riv/klicoveSlovo	formalin-fixed paraffin-embedded tissue gene expression real-time quantitative polymerase chain reaction TaqMan probe reverse transcription
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[5A6652E88B7B]
http://linked.open...i/riv/nazevZdroje	Diagnostic molecular pathology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	18
http://linked.open...iv/tvurceVysledku	Forsterová, Kristina Trněný, Marek Votavová, Hana Stříteský, Jan Velenská, Zuzana
http://linked.open...ain/vavai/riv/wos	000269378900008
http://linked.open...n/vavai/riv/zamer	Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu
issn	1052-9551
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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