About: Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues

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About: Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We present a rapid and sensitive LC-MS/MS method for SAM and SAH determination in mice tissues. The method is based on chromatographic separation on a Hypercarb column filled with porous graphitic carbon stationary phase. Sufficient retention of SAM and SAH on the chromatographic packing allows simple sample preparation protocol avoiding solid phase extraction step. No significant matrix effects were observed by analysing the tissue extracts on LC-MS/MS. We present a rapid and sensitive LC-MS/MS method for SAM and SAH determination in mice tissues. The method is based on chromatographic separation on a Hypercarb column filled with porous graphitic carbon stationary phase. Sufficient retention of SAM and SAH on the chromatographic packing allows simple sample preparation protocol avoiding solid phase extraction step. No significant matrix effects were observed by analysing the tissue extracts on LC-MS/MS. (en)
Title	Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues (en)
skos:prefLabel	Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues (en)
skos:notation	RIV/00216208:11110/09:4121!RIV10-MSM-11110___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021620806)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Krijt, Jakub Kožich, Viktor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 1. lékařská fakulta
http://linked.open...dnocenehoVysledku	309936
http://linked.open...ai/riv/idVysledku	RIV/00216208:11110/09:4121
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	S-Adenosylmethionine; S-Adenosylhomocysteine; Mass spectrometry; Liquid chromatography; Mouse tissues; cystathionine beta-synthase; performance liquid-chromatography; tandem mass-spectrometry; adenosyl-l-methionine; plasma homocysteine; cerebrospinal-fluid; methylation ratio; deficient; mouse; hydrolase (en)
http://linked.open.../riv/klicoveSlovo	S-Adenosylmethionine Liquid chromatography performance liquid-chromatography Mouse tissues S-Adenosylhomocysteine adenosyl-l-methionine deficient methylation ratio Mass spectrometry hydrolase cystathionine beta-synthase tandem mass-spectrometry plasma homocysteine mouse cerebrospinal-fluid
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[3AB1F02CBD98]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	877
http://linked.open...iv/tvurceVysledku	Kožich, Viktor Krijt, Jakub Duta, A.
http://linked.open...ain/vavai/riv/wos	000268040400012
http://linked.open...n/vavai/riv/zamer	Molekulární biologie a patologie buňky za normy a u vybraných klinicky závažných patologických procesů
issn	1570-0232
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	11110

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