About: Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation

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About: Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1016/j.electacta.2014.10.125
Description	His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, a-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedicine. His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, a-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedicine. (en)
Title	Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation (en)
skos:prefLabel	Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation Effect of His6-tagging of anterior gradient 2 protein on its electro-oxidation (en)
skos:notation	RIV/00209805:_____/14:#0000567!RIV15-GA0-00209805
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0101), P(GA13-00956S)
http://linked.open...iv/cisloPeriodika	4 November
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ďurech, Michal Hrstka, Roman Paleček, Emil Vojtěšek, Bořivoj
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	13332
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/14:#0000567
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	anterior gradient 2 oncoprotein; His-tagged proteins; carbon electrode; histidine; tyrosine and tryptophan oxidation (en)
http://linked.open.../riv/klicoveSlovo	His-tagged proteins anterior gradient 2 oncoprotein tyrosine and tryptophan oxidation histidine carbon electrode
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[B3151DCC3F38]
http://linked.open...i/riv/nazevZdroje	Electrochimica acta
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Emerging roles for the proteins of Anterior Gradient family in cancer development Regional Centre for Applied Molecular Oncology (RECAMO)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	150
http://linked.open...iv/tvurceVysledku	Hrstka, Roman Vojtěšek, Bořivoj Paleček, Emil Ďurech, Michal
http://linked.open...ain/vavai/riv/wos	000346185000028
issn	0013-4686
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.electacta.2014.10.125

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