About: Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers

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About: Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S1388248113001410
Description	Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and 2,2´-bipyridine, Os(VI)bipy, specifically binding to the 3´-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labelingstep with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture ofother noncomplementary miRNAs was possible. Recently, altered expression levels of microRNAs (miRNAs) - short noncoding RNA molecules which bind to mRNAs and thus regulate gene expression - were observed in many cancer cells. miRNA expression profiling is therefore of great interest, but current standard methods are still considered relatively laborious and expensive. Electrochemistry has a potential to become quick and inexpensive alternative. Here, we describe modification of miRNA with an electroactive complex composed of six-valent osmium and 2,2´-bipyridine, Os(VI)bipy, specifically binding to the 3´-end of the ribose, which is detectable at hanging mercury drop electrode at femtomole level due to an electrocatalytic nature of a resulting signal. By combining miRNA labelingstep with magnetic beads-based hybridization assay, detection of specific miRNA sequence from a mixture ofother noncomplementary miRNAs was possible. (en)
Title	Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers (en)
skos:prefLabel	Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers Os(VI)bipy-based electrochemical assay for detection of specific microRNAs as potential cancer biomarkers (en)
skos:notation	RIV/00209805:_____/13:#0000415!RIV14-GA0-00209805
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED2.1.00/03.0101), P(GAP301/11/2055), P(GBP206/12/G151), Z(AV0Z50040702)
http://linked.open...iv/cisloPeriodika	August
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hrstka, Roman Paleček, Emil Vojtěšek, Bořivoj Bartošík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykův onkologický ústav
http://linked.open...dnocenehoVysledku	94808
http://linked.open...ai/riv/idVysledku	RIV/00209805:_____/13:#0000415
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microRNA; cancer diagnostics; electrochemistry; mercury electrodes (en)
http://linked.open.../riv/klicoveSlovo	cancer diagnostics electrochemistry mercury electrodes microRNA
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[BC8934C49E90]
http://linked.open...i/riv/nazevZdroje	Electrochemistry Communications
http://linked.open...in/vavai/riv/obor	CG
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics Regional Centre for Applied Molecular Oncology (RECAMO) New methods of analysis of proteins and their glycosylation in cancer - combination of electrochemistry, microfludic biosensors and mass spectrometry.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	33
http://linked.open...iv/tvurceVysledku	Hrstka, Roman Vojtěšek, Bořivoj Paleček, Emil Bartošík, Martin
http://linked.open...ain/vavai/riv/wos	000323024600014
http://linked.open...n/vavai/riv/zamer	Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions
issn	1388-2481
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.elecom.2013.04.009

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